MGI phenotypes (allele matching)
B6N.129-Inca1tm1Cmt/Ieg
| Status | Available to order |
| EMMA ID | EM:07365 |
| Citation information | RRID:IMSR_EM:07365 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6N.129-Inca1tm1Cmt/Ieg |
| Alternative name | Inca1-Knockout |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Inca1tm1Cmt |
| Gene/Transgene symbol | Inca1 |
Information from provider
| Provider | Carsten Mueller-Tidow |
| Provider affiliation | Departmen of Medicine A, Molecular Hematology and Oncology, University of Muenster |
| Genetic information | To generate an Inca1-null mouse, we electroporated 129/Sv ES cells with a targeting construct, resulting in the replacement of the Inca1 exons 3 to 7 with an IRES-lacZ cassette and the neomycin resistance gene flanked by loxP sites by homologous recombination. |
| Phenotypic information | Inca1-/- mice show increased CDK2 activity in spleen and altered spleen architecture. Hematopoietic and leukemic stem cells are affected in their function in absence of Inca1 (manuscript in preparation). |
| Breeding history | Inca1-knockout mice were backcrossed for at least 6 generations to C57BL/6N. Currently, the strain is maintained by breeding heterozygous male with heterozygous females or by breeding homozygous males or females with wild-type mice. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | heterozygous C57BL/6N males, wild-type C57BL/6NTac females |
| Stage of embryos | 2-cell |
Disease and phenotype information
Literature references
- Inhibitor of cyclin-dependent kinase (CDK) interacting with cyclin A1 (INCA1) regulates proliferation and is repressed by oncogenic signaling.;Bäumer Nicole, Tickenbrock Lara, Tschanter Petra, Lohmeyer Lisa, Diederichs Sven, Bäumer Sebastian, Skryabin Boris V, Zhang Feng, Agrawal-Singh Shuchi, Köhler Gabriele, Berdel Wolfgang E, Serve Hubert, Koschmieder Steffen, Müller-Tidow Carsten, ;2011;The Journal of biological chemistry;286;28210-22; 21540187
- The inhibitor of growth protein 5 (ING5) depends on INCA1 as a co-factor for its antiproliferative effects.;Zhang Feng, Bäumer Nicole, Rode Miriam, Ji Ping, Zhang Tao, Berdel Wolfgang E, Müller-Tidow Carsten, ;2011;PloS one;6;e21505; 21750715