SJLJ.129S-Lgals1tm1Rob/H

Status

Available to order

EMMA IDEM:07421
Citation informationRRID:IMSR_EM:07421 

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International strain nameSJLJ.129S-Lgals1tm1Rob/H
Alternative nameSJL/J Lgals1tm1Rob
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolLgals1tm1Rob
Gene/Transgene symbolLgals1

Information from provider

ProviderFrancis Szele
Provider affiliationDepartment of anatomy physiology and genetics, University of Oxford
Genetic informationLgals1 (Gal1) -/- 129Sv mice were generated as in Poirier F and Robertson E (1993) Development v. 119, p. 1229 - 1236. Exon 2, encoding the carbohydrate-binding domain, was replaced with a neomycin selection cassette. Western blot analysis and immunostaining of sections showed an absence of encoded protein in muscle tissue obtained from adult homozygous mutant mice. To change the background of this strain an homozygous Gal1-/- 129Sv was crossbred with a SJL/J mouse. Backcrosses 2-10 were heterozygous Gal1+/- SJL/J with SJL/J; heterozygous breeding was alternated each generation with male and female.
Phenotypic informationThese mice are susceptible to Theiler's Murine Encephalomyelitis Virus infection and can be used to model primary progressive multiple sclerosis. During the backcrossing there was high mortality rate amongst litters and very poor breeding due to aversion to other mouse strain pheromones. SJL/J males also exhibited significant aggression often resulting in amyloidosis (sequel of social submissiveness and consequent wounds) in female breeding partners, which were often very severe and required culling.
Breeding historyA homozygous Gal1-/- 129Sv was crossbred with an SJL/J mouse. Backcrosses 2-10 were heterozygous Gal1+/- SJL/J with SJL/J; heterozygous breeding was alternated each generation with male and female.
References
  • Normal development of mice carrying a null mutation in the gene encoding the L14 S-type lectin.;Poirier F, Robertson E J, ;1993;Development (Cambridge, England);119;1229-36; 8306885
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

MGI phenotypes (gene matching)
  • delayed muscle development / MGI
  • abnormal dorsal root ganglion morphology / MGI
  • abnormal sensory neuron morphology / MGI
  • abnormal nociceptor morphology / MGI
  • abnormal mechanoreceptor morphology / MGI
  • abnormal neuromuscular synapse morphology / MGI
  • increased anxiety-related response / MGI
  • increased thermal nociceptive threshold / MGI
  • abnormal social/conspecific interaction / MGI
  • abnormal cytokine secretion / MGI
  • abnormal postnatal subventricular zone morphology / MGI
  • increased susceptibility to experimental autoimmune encephalomyelitis / MGI
  • abnormal olfactory nerve morphology / MGI
  • homeostasis/metabolism phenotype / MGI
  • behavior/neurological phenotype / MGI
  • reproductive system phenotype / MGI
  • abnormal olfactory sensory neuron morphology / MGI
  • increased interferon-gamma secretion / MGI
  • increased interleukin-2 secretion / MGI
  • mortality/aging / MGI

Literature references

  • Normal development of mice carrying a null mutation in the gene encoding the L14 S-type lectin.;Poirier F, Robertson E J, ;1993;Development (Cambridge, England);119;1229-36; 8306885

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Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

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  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
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