STOCK Lgals3^tm1Poi/H

Status	Available to order
EMMA ID	EM:07422
Citation information	RRID:IMSR_EM:07422 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	STOCK Lgals3^tm1Poi/H
Alternative name	SJL/J Lgals3tm1Poi
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Lgals3^tm1Poi
Gene/Transgene symbol	Lgals3

Information from provider

Provider	Francis Szele
Provider affiliation	Department of anatomy physiology and genetics, University of Oxford
Genetic information	Lgals3 (Gal3) -/- 129Sv mice were generated as in Colnot, C., Fowlis, D., Ripoche, M. A., Bouchaert, I. and Poirier, F. (1998; Embryonic implantation in galectin 1/galectin 3 double mutant mice. Dev. Dyn. 211, 306-313), as follows: 3.7kb of sequence, encompassing exon 2 through to 4, was replaced by a neomycin selection cassette. The construct was electroporated into WW6 embryonic stem cells. Correctly targeted ES cells were injected into outbred MF1 blastocysts and implanted into pseudo-pregnant dames. The resultant germline chimeric male offspring were crossed with female 129/Sv mice to produce heterozygotes for the recombined allele. To generate SJL/J Lgals3 KO mice, an homozygous Gal3-/- 129/Sv was crossbred with an SJL/J mouse. Backcrosses 2-10 were heterozygous Gal3+/- SJL/J bred with SJL/J, heterozygous breeding was alternated each generation with male and female.
Phenotypic information	These mice are susceptible to Theiler's Murine Encephalomyelitis Virus infection and can be used to model primary progressive multiple sclerosis. During the backcrossing there was high mortality rate amongst litters and very poor breeding due to aversion to other mouse strain pheromones. SJL/J males also exhibited significant aggression often resulting in amyloidosis (sequel of social submissiveness and consequent wounds) in female breeding partners, which were often severe and required culling.
Breeding history	An homozygous Gal3-/- 129/Sv was crossbred with an SJL/J mouse. Backcrosses 2-10 were heterozygous Gal3+/- SJL/J bred with SJL/J, heterozygous breeding was alternated each generation with male and female.
References	Embryonic implantation in galectin 1/galectin 3 double mutant mice.;Colnot C, Fowlis D, Ripoche M A, Bouchaert I, Poirier F, ;1998;Developmental dynamics : an official publication of the American Association of Anatomists;211;306-13; 9566950
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

MGI phenotypes (gene matching)

increased bone mineral density / MGI
abnormal long bone hypertrophic chondrocyte zone / MGI
decreased monocyte cell number / MGI
decreased granulocyte number / MGI
abnormal liver morphology / MGI
abnormal hepatocyte morphology / MGI
increased body weight / MGI
aggression towards inanimate objects / MGI
abnormal stationary movement / MGI
hypoactivity / MGI
focal hepatic necrosis / MGI
impaired macrophage phagocytosis / MGI
brain inflammation / MGI
liver inflammation / MGI
lung inflammation / MGI
abnormal kidney physiology / MGI
abnormal dendritic cell physiology / MGI
increased susceptibility to bacterial infection / MGI
abnormal CD8-positive, alpha-beta cytotoxic T cell morphology / MGI
abnormal leukocyte physiology / MGI
abnormal macrophage physiology / MGI
increased IgG level / MGI
abnormal social/conspecific interaction / MGI
abnormal monocyte morphology / MGI
hepatic steatosis / MGI
increased circulating alanine transaminase level / MGI
increased liver weight / MGI
abnormal long bone epiphyseal plate morphology / MGI
peritoneal inflammation / MGI
small intestinal inflammation / MGI
increased hepatocellular carcinoma incidence / MGI
liver fibrosis / MGI
decreased width of hypertrophic chondrocyte zone / MGI
oxidative stress / MGI
impaired macrophage chemotaxis / MGI
decreased macrophage cell number / MGI
increased eosinophil cell number / MGI
decreased lymphocyte cell number / MGI
abnormal response to infection / MGI
increased susceptibility to parasitic infection / MGI
sepsis / MGI
decreased susceptibility to injury / MGI
reproductive system phenotype / MGI
abnormal interferon secretion / MGI
increased tumor necrosis factor secretion / MGI
increased interleukin-12b secretion / MGI
increased interleukin-6 secretion / MGI
impaired neutrophil recruitment / MGI
increased liver triglyceride level / MGI
impaired cued conditioning behavior / MGI
increased sensitivity to induced morbidity/mortality / MGI
increased total body fat amount / MGI
mortality/aging / MGI
integument phenotype / MGI
increased macrophage apoptosis / MGI
Mallory bodies / MGI
abnormal circadian behavior / MGI

Literature references

Embryonic implantation in galectin 1/galectin 3 double mutant mice.;Colnot C, Fowlis D, Ripoche M A, Bouchaert I, Poirier F, ;1998;Developmental dynamics : an official publication of the American Association of Anatomists;211;306-13; 9566950

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Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
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STOCK Lgals3tm1Poi/H