B6;129P2-Adam10^tm2Psa/Ph

Status	Available to order
EMMA ID	EM:07449
Citation information	RRID:IMSR_EM:07449 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6;129P2-Adam10^tm2Psa/Ph
Alternative name	Adam10flox
Strain type	Targeted Mutant Strains : Conditional mutation
Allele/Transgene symbol	Adam10^tm2Psa
Gene/Transgene symbol	Adam10

Information from provider

Provider	Paul Saftig
Provider affiliation	Institute of Biochemistry, Christian-Albrechts-University Kiel
Genetic information	A mouse cosmid clone containing the 5' region of the Adam10 gene with exon 2 was isolated from a 129/Ola cosmid library. For construction of the targeting vector, an 8.2 kb KpnI-PstI DNA restriction fragment of Adam10 covering exon 2 was subcloned into the plasmid vector pUC-18. The hygromycin B resistance gene, driven by PGK promoter flanked with two flippase recognition target sequences, one loxP sequence downstream of the hygromycin B resistance gene, was inserted into NotI-ApaI site 5' upstream of exon2 into intron1. A second loxP sequence was inserted into the SphI-SpeI site in intron 2. The targeting vector was linearized and introduced into ES cell line E14 129P2/OlaHsd. Mutated ES cell lines were microinjected into blastocysts of C57BL/6J mice.
Phenotypic information	Adam10floxed mice do not show specific phenotype, they have normal habitus and appearance.
Breeding history	After ES cell injection into 129/SvEv Adam10 flox mice were backcrossed 3 times onto C57BL/6N background. Colony is now maintained on C57BL/6NCrl background.
References	The disintegrin/metalloproteinase ADAM10 is essential for the establishment of the brain cortex.;Jorissen Ellen, Prox Johannes, Bernreuther Christian, Weber Silvio, Schwanbeck Ralf, Serneels Lutgarde, Snellinx An, Craessaerts Katleen, Thathiah Amantha, Tesseur Ina, Bartsch Udo, Weskamp Gisela, Blobel Carl P, Glatzel Markus, De Strooper Bart, Saftig Paul, ;2010;The Journal of neuroscience : the official journal of the Society for Neuroscience;30;4833-44; 20371803
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Institute of Molecular Genetics, Prague, Czech Republic

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Reticulate acropigmentation of Kitamura / Orphanet_178307

MGI phenotypes (gene matching)

failure of vascular branching / MGI
distended pericardium / MGI
abnormal pontine flexure morphology / MGI
abnormal somite development / MGI
decreased embryo size / MGI
abnormal visceral yolk sac morphology / MGI
abnormal umbilical cord morphology / MGI
abnormal neural tube morphology / MGI
no abnormal phenotype detected / MGI
abnormal vitelline vasculature morphology / MGI
abnormal forebrain development / MGI
small first pharyngeal arch / MGI
abnormal paraxial mesoderm morphology / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
delayed heart development / MGI
absent intersomitic vessels / MGI

Literature references

The disintegrin/metalloproteinase ADAM10 is essential for the establishment of the brain cortex.;Jorissen Ellen, Prox Johannes, Bernreuther Christian, Weber Silvio, Schwanbeck Ralf, Serneels Lutgarde, Snellinx An, Craessaerts Katleen, Thathiah Amantha, Tesseur Ina, Bartsch Udo, Weskamp Gisela, Blobel Carl P, Glatzel Markus, De Strooper Bart, Saftig Paul, ;2010;The Journal of neuroscience : the official journal of the Society for Neuroscience;30;4833-44; 20371803

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Example health report
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Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

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B6;129P2-Adam10tm2Psa/Ph