IMPC phenotypes (gene matching)
- hyperactivity / IMPC
CD1.129-Sigmar1tm1Lmon/Cnbc
| Status | Available to order |
| EMMA ID | EM:07742 |
| Citation information | RRID:IMSR_EM:07742 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | CD1.129-Sigmar1tm1Lmon/Cnbc |
| Alternative name | STOCK CD1 Sigmar1 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Sigmar1tm1Lmon |
| Gene/Transgene symbol | Sigmar1 |
Information from provider
| Provider | Jose Miguel Vela |
| Provider affiliation | Drug Discovery & Preclinical Development, ESTEVE |
| Genetic information | The mouse Sigma1 receptor gene (Sigmar1) was disrupted in mouse R1 ES cells using a replacement vector-type approach. An in-frame lacZ gene and neo cassette from the targeting vector pHR53TK were inserted after the first 4 codons deleting subsequent 1907 bases via homologous recombination. Northern and Western blot analysis confirmed the absence of mRNA and protein expression in homozygous mice. |
| Phenotypic information | Homozygous mutant mice are viable and fertile. Significant decrease in hypermotility response is observed upon challenging mutant mice with (+)SKF-10047. Tonic pain induced by formalin is altered (formalin-induced pain is reduced by approximately 55%) in mutant mice, in comparison to wild-type animals. Similarly, neuropathic pain is reduced in Sigma1r mutant mice. Sigma1r is essential for capsaicin-induced mechanical hypersensitivity. |
| Breeding history | Germ-line transmitting chimeras (males), derived from hybrid 129 R1 ES cells, were bred to CD1 females. Heterozygous individuals carrying the Sigmar1 targeted allele were selected, bred and backcrossed to CD1 outbred albino mice for more than 10 generations. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous CD-1 males, wild-type CD-1 females |
| Stage of embryos | 8-cell |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Distal hereditary motor neuropathy, Jerash type / Orphanet_139552
- Juvenile amyotrophic lateral sclerosis / Orphanet_300605
MGI phenotypes (allele matching)
- hypoactivity / MGI
Literature references
- Generation and phenotypic analysis of sigma receptor type I (sigma 1) knockout mice.;Langa Francina, Codony Xavier, Tovar Victoria, Lavado Alfonso, Giménez Estela, Cozar Patricia, Cantero Marta, Dordal Albert, Hernández Enric, Pérez Raquel, Monroy Xavier, Zamanillo Daniel, Guitart Xavier, Montoliu Lluís, ;2003;The European journal of neuroscience;18;2188-96; 14622179
- Formalin-induced pain is reduced in sigma(1) receptor knockout mice.;Cendán Cruz Miguel, Pujalte José Miguel, Portillo-Salido Enrique, Montoliu Lluís, Baeyens José M, ;2005;European journal of pharmacology;511;73-4; 15777781
- Induction of hypothermic conditions associated with increased micronuclei formation in sigma-1 receptor knockout mice after administration of the antipsychotic compound E-5842.;Guzmán Antonio, Marín Ana-Paz, García Concepción, Fernández de Henestrosa Antonio R, Ruiz María Teresa, Tortajada Araceli, Marcos Ricard, ;2008;Environmental and molecular mutagenesis;49;727-33; 18800345
- Sigma-1 receptors regulate activity-induced spinal sensitization and neuropathic pain after peripheral nerve injury.;Puente Beatriz de la, Nadal Xavier, Portillo-Salido Enrique, Sánchez-Arroyos Ricard, Ovalle Sergio, Palacios Gabriel, Muro Asunción, Romero Luz, Entrena José Manuel, Baeyens José Manuel, López-García José Antonio, Maldonado Rafael, Zamanillo Daniel, Vela José Miguel, ;2009;Pain;145;294-303; 19505761
- Selective sigma-1 (sigma1) receptor antagonists: emerging target for the treatment of neuropathic pain.;Díaz José Luis, Zamanillo Daniel, Corbera Jordi, Baeyens José Manuel, Maldonado Rafael, Pericàs Miquel Angel, Vela José Miguel, Torrens Antoni, ;2009;Central nervous system agents in medicinal chemistry;9;172-83; 20021351
- Sigma-1 receptor antagonism as opioid adjuvant strategy: enhancement of opioid antinociception without increasing adverse effects.;Vidal-Torres Alba, de la Puente Beatriz, Rocasalbas Maria, Touriño Clara, Bura Simona Andreea, Fernández-Pastor Begoña, Romero Luz, Codony Xavier, Zamanillo Daniel, Buschmann Helmut, Merlos Manuel, Baeyens José Manuel, Maldonado Rafael, Vela José Miguel, ;2013;European journal of pharmacology;711;63-72; 23632394