IMPC phenotypes (gene matching)
B6;129-Cdkn1ctm1Bbd/Cnbc
| Status | Available to order |
| EMMA ID | EM:08025 |
| Citation information | RRID:IMSR_EM:08025 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;129-Cdkn1ctm1Bbd/Cnbc |
| Alternative name | Cdkn1c |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Cdkn1ctm1Bbd |
| Gene/Transgene symbol | Cdkn1c |
Information from provider
| Provider | Mariano Barbacid |
| Provider affiliation | Molecular Oncology, Centro Nacional de Investigaciones Oncologicas |
| Genetic information | Deletion of 1.1 kb of genomic sequence that includes the Cdkn1c first exon and part of the second exon, encoding amino acid residues 1-181 of the protein. No protein was detected in Western blots of lysates of primary embryo fibroblasts derived from homozygous mutant mice. |
| Phenotypic information | Homozygous:Most Cdkn1c (Kip2 or p57) null mice die after birth and display severe developmental defects with varying degrees of penetrance. Heterozygous mice that inherit a maternal, but not a paternal, targeted allele exhibit similar deficiencies and neonatal death. Developmental defects of Cdkn1c mutant mice include cleft palate and gastrointestinal abnormalities. Most Cdkn1c mutant mice have short limbs, a defect attributable to abnormal endochondral ossification caused by delayed cell cycle exit during chondrocyte differentiation.Heterozygous:Heterozygous mice that inherit a maternal, but not a paternal, targeted allele exhibit similar deficiencies and neonatal death. |
| Breeding history | Mutation introduced in R1 ES cells. Chimeras were bred with C57BL/6J females. Heterozygous mice were bred among themselves to generate homozygous mice or to C57BL/6J animals to propagate the targeted allele. Maintained currently in mixed background: 129S1/Sv, 129X1/SvJ and C57BL/6 (at least 80%). |
| References |
|
| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous males, wild-type mixed 129/SvJ, C57BL/6 females |
| Stage of embryos | 4/8-cell |
Disease and phenotype information
MGI phenotypes (allele matching)
- cleft palate / MGI
- abnormal intestine morphology / MGI
- short limbs / MGI
- abnormal suckling behavior / MGI
- paternal imprinting / MGI
- absent gastric milk in neonates / MGI
- neonatal lethality, incomplete penetrance / MGI
- lethality throughout fetal growth and development, incomplete penetrance / MGI
- abnormal long bone epiphysis morphology / MGI
- absent jejunum / MGI
- abnormal midbrain morphology / MGI
- abnormal ileum morphology / MGI
- abnormal digestive system development / MGI
- abnormal soft palate morphology / MGI
- abnormal duodenum morphology / MGI
- delayed endochondral bone ossification / MGI
- abnormal hard palate morphology / MGI
- abnormal bone ossification / MGI
- meteorism / MGI
- palatal shelves fail to meet at midline / MGI
- increased midbrain apoptosis / MGI
- abnormal digestive system morphology / MGI
- abnormal skeleton development / MGI
- abnormal limb bone morphology / MGI
- abnormal craniofacial bone morphology / MGI
Literature references
- Ablation of the CDK inhibitor p57Kip2 results in increased apoptosis and delayed differentiation during mouse development.;Yan Y, Frisén J, Lee M H, Massagué J, Barbacid M, ;1997;Genes & development;11;973-83; 9136926
- p57(Kip2) cooperates with Nurr1 in developing dopamine cells.;Joseph Bertrand, Wallén-Mackenzie Asa, Benoit Gérard, Murata Takashi, Joodmardi Eliza, Okret Sam, Perlmann Thomas, ;2003;Proceedings of the National Academy of Sciences of the United States of America;100;15619-24; 14671317
- Identification and characterisation of imprinted genes in the mouse.;Peters Jo, Beechey Colin, ;2004;Briefings in functional genomics & proteomics;2;320-33; 15163367