B6.129-Cdkn2btm1Bbd/Cnbc

Status

Available to order

EMMA IDEM:08026
Citation informationRRID:IMSR_EM:08026 

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International strain nameB6.129-Cdkn2btm1Bbd/Cnbc
Alternative nameCdkn2b or p15Ink4b KO
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolCdkn2btm1Bbd
Gene/Transgene symbolCdkn2b

Information from provider

ProviderMariano Barbacid
Provider affiliationMolecular Oncology, Centro Nacional de Investigaciones Oncologicas
Genetic informationExon 2, encoding amino acids 44-130, was replaced by a neomycin cassette. No protein was detectable in Western blots of immunoprecipitates of MEF lysates derived from homozygous mutant embryos.
Phenotypic informationHomozygous:
Some mice die between 9 and 18 months of age; these mice have no evidence of tumors but have severe extra-medullary hematopoiesis. Abnormal lymphocyte morphology, spleen and lymph nodes hyperplasia, 8.2% incidence of tumors. MEFs exhibit a higher proliferation rate and they are susceptible to Ras-induced transformation.

Heterozygous:
Heterozygous mice are viable
Breeding historyChimeras were bred with C57BL/6J females. Heterozygous mice were bred among themselves to generate homozygous mice or to C57BL/6J animals to propagate the targeted allele. Mice were bred for more than 10 generations with C57BL/6J mice before freezing.
References
  • Limited overlapping roles of P15(INK4b) and P18(INK4c) cell cycle inhibitors in proliferation and tumorigenesis.;Latres E, Malumbres M, Sotillo R, Martín J, Ortega S, Martín-Caballero J, Flores J M, Cordón-Cardo C, Barbacid M, ;2000;The EMBO journal;19;3496-506; 10880462
  • Invasive melanoma in Cdk4-targeted mice.;Sotillo R, García J F, Ortega S, Martin J, Dubus P, Barbacid M, Malumbres M, ;2001;Proceedings of the National Academy of Sciences of the United States of America;98;13312-7; 11606789
  • Hypermethylation of the Ink4b locus in murine myeloid leukemia and increased susceptibility to leukemia in p15(Ink4b)-deficient mice.;Wolff Linda, Garin Matthew T, Koller Richard, Bies Juraj, Liao Wei, Malumbres Marcos, Tessarollo Lino, Powell Douglas, Perella Christine, ;2003;Oncogene;22;9265-74; 14681685
  • p21Cip1 and p27Kip1 act in synergy to alter the sensitivity of naive T cells to TGF-beta-mediated G1 arrest through modulation of IL-2 responsiveness.;Wolfraim Lawrence A, Walz Thomas M, James Zakiya, Fernandez Tania, Letterio John J, ;2004;Journal of immunology (Baltimore, Md. : 1950);173;3093-102; 15322169
  • The Rgr oncogene induces tumorigenesis in transgenic mice.;Jiménez María, Pérez de Castro Ignacio, Benet Marta, García Juan F, Inghirami Giorgio, Pellicer Angel, ;2004;Cancer research;64;6041-9; 15342385
  • Loss of the tumor suppressor p15Ink4b enhances myeloid progenitor formation from common myeloid progenitors.;Rosu-Myles Michael, Taylor Barbara J, Wolff Linda, ;2007;Experimental hematology;35;394-406; 17309820
  • p15(INK4b) plays a crucial role in murine lymphoid development and tumorigenesis.;Osei-Sarfo Kwame, de Castro Ignacio Perez, Pellicer Angel, ;2012;Carcinogenesis;33;708-13; 22227036
  • The tumor suppressor p15Ink4b regulates the differentiation and maturation of conventional dendritic cells.;Fares Joanna, Koller Richard, Humeniuk Rita, Wolff Linda, Bies Juraj, ;2012;Blood;119;5005-15; 22461492
  • Congenital asplenia in mice and humans with mutations in a Pbx/Nkx2-5/p15 module.;Koss Matthew, Bolze Alexandre, Brendolan Andrea, Saggese Matilde, Capellini Terence D, Bojilova Ekaterina, Boisson Bertrand, Prall Owen W J, Elliott David A, Solloway Mark, Lenti Elisa, Hidaka Chisa, Chang Ching-Pin, Mahlaoui Nizar, Harvey Richard P, Casanova Jean-Laurent, Selleri Licia, ;2012;Developmental cell;22;913-26; 22560297
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivingheterozygous C57BL/6J males

Disease and phenotype information

MGI phenotypes (allele matching)
  • extramedullary hematopoiesis / MGI
  • lymphoid hyperplasia / MGI
  • abnormal spleen morphology / MGI
  • spleen hyperplasia / MGI
  • increased tumor incidence / MGI
  • increased sarcoma incidence / MGI
  • premature death / MGI
  • abnormal lymph node secondary follicle morphology / MGI
  • abnormal spleen marginal zone morphology / MGI
  • abnormal lymphocyte morphology / MGI
  • abnormal cell physiology / MGI
  • lymph node hyperplasia / MGI
  • increased mature B cell number / MGI
  • abnormal lymph node germinal center morphology / MGI
  • increased adenocarcinoma incidence / MGI
  • increased fibroblast proliferation / MGI
MGI phenotypes (gene matching)
  • extramedullary hematopoiesis / MGI
  • lymphoid hyperplasia / MGI
  • abnormal spleen morphology / MGI
  • spleen hyperplasia / MGI
  • increased tumor incidence / MGI
  • increased sarcoma incidence / MGI
  • premature death / MGI
  • abnormal lymph node secondary follicle morphology / MGI
  • abnormal spleen marginal zone morphology / MGI
  • abnormal lymphocyte morphology / MGI
  • no phenotypic analysis / MGI
  • abnormal cell physiology / MGI
  • lymph node hyperplasia / MGI
  • increased mature B cell number / MGI
  • abnormal lymph node germinal center morphology / MGI
  • increased adenocarcinoma incidence / MGI
  • increased fibroblast proliferation / MGI

Literature references

  • Limited overlapping roles of P15(INK4b) and P18(INK4c) cell cycle inhibitors in proliferation and tumorigenesis.;Latres E, Malumbres M, Sotillo R, Martín J, Ortega S, Martín-Caballero J, Flores J M, Cordón-Cardo C, Barbacid M, ;2000;The EMBO journal;19;3496-506; 10880462
  • Invasive melanoma in Cdk4-targeted mice.;Sotillo R, García J F, Ortega S, Martin J, Dubus P, Barbacid M, Malumbres M, ;2001;Proceedings of the National Academy of Sciences of the United States of America;98;13312-7; 11606789
  • Hypermethylation of the Ink4b locus in murine myeloid leukemia and increased susceptibility to leukemia in p15(Ink4b)-deficient mice.;Wolff Linda, Garin Matthew T, Koller Richard, Bies Juraj, Liao Wei, Malumbres Marcos, Tessarollo Lino, Powell Douglas, Perella Christine, ;2003;Oncogene;22;9265-74; 14681685
  • p21Cip1 and p27Kip1 act in synergy to alter the sensitivity of naive T cells to TGF-beta-mediated G1 arrest through modulation of IL-2 responsiveness.;Wolfraim Lawrence A, Walz Thomas M, James Zakiya, Fernandez Tania, Letterio John J, ;2004;Journal of immunology (Baltimore, Md. : 1950);173;3093-102; 15322169
  • The Rgr oncogene induces tumorigenesis in transgenic mice.;Jiménez María, Pérez de Castro Ignacio, Benet Marta, García Juan F, Inghirami Giorgio, Pellicer Angel, ;2004;Cancer research;64;6041-9; 15342385
  • Loss of the tumor suppressor p15Ink4b enhances myeloid progenitor formation from common myeloid progenitors.;Rosu-Myles Michael, Taylor Barbara J, Wolff Linda, ;2007;Experimental hematology;35;394-406; 17309820
  • p15(INK4b) plays a crucial role in murine lymphoid development and tumorigenesis.;Osei-Sarfo Kwame, de Castro Ignacio Perez, Pellicer Angel, ;2012;Carcinogenesis;33;708-13; 22227036
  • The tumor suppressor p15Ink4b regulates the differentiation and maturation of conventional dendritic cells.;Fares Joanna, Koller Richard, Humeniuk Rita, Wolff Linda, Bies Juraj, ;2012;Blood;119;5005-15; 22461492
  • Congenital asplenia in mice and humans with mutations in a Pbx/Nkx2-5/p15 module.;Koss Matthew, Bolze Alexandre, Brendolan Andrea, Saggese Matilde, Capellini Terence D, Bojilova Ekaterina, Boisson Bertrand, Prall Owen W J, Elliott David A, Solloway Mark, Lenti Elisa, Hidaka Chisa, Chang Ching-Pin, Mahlaoui Nizar, Harvey Richard P, Casanova Jean-Laurent, Selleri Licia, ;2012;Developmental cell;22;913-26; 22560297

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