IMPC phenotypes (gene matching)
B6.129P2-Lamp1tm1Psa/Ph
| Status | Available to order |
| EMMA ID | EM:08121 |
| Citation information | RRID:IMSR_EM:08121 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129P2-Lamp1tm1Psa/Ph |
| Alternative name | Lamp1 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Lamp1tm1Psa |
| Gene/Transgene symbol | Lamp1 |
Information from provider
| Provider | Paul Saftig |
| Provider affiliation | Institute of Biochemistry, Christian-Albrechts-University Kiel |
| Genetic information | For construction of a targeting vector, a 5.3-kbp KpnI DNA restriction fragment of Lamp1 covering exons 2 and 3 was derived from 129Sv phage library and subcloned. The neo expression cassette was inserted as BamHI DNA restriction fragment into a BglII restriction site located in exon 3 of the KpnI fragment (nucleotide position 323 of the Lamp1 cDNA; amino acid 107 of 382). The insertion of the neo cassette introduces a premature translational stop codon into the open reading frame of the Lamp1 gene. Additionally, for negative selection a thymidine kinase cassette was inserted at the 3' site of the KpnI fragment. Linearized vector was introduced into E14.1 ES cells. Correctly targeted clones were injected into C57BL/6J blastocysts. |
| Phenotypic information | Homozygous:Homozygous mice do not exhibit gross changes in the phenotype. Focusing on the brain, Lamp1-deficient mice show altered cathepsin D immunoreactivity and mild astrogliosis.Heterozygous:Heterozygous animals appear normal, like wild-type animals. |
| Breeding history | Backcrossed, N=8 |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institute of Molecular Genetics, Prague, Czech Republic |
Disease and phenotype information
MGI phenotypes (gene matching)
- no abnormal phenotype detected / MGI
Literature references
- Normal lysosomal morphology and function in LAMP-1-deficient mice.;Andrejewski N, Punnonen E L, Guhde G, Tanaka Y, Lüllmann-Rauch R, Hartmann D, von Figura K, Saftig P, ;1999;The Journal of biological chemistry;274;12692-701; 10212251