MGI phenotypes (allele matching)
B6.129X1-Mmp28tm1Mani/Ph
| Status | Available to order |
| EMMA ID | EM:08133 |
| Citation information | RRID:IMSR_EM:08133 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129X1-Mmp28tm1Mani/Ph |
| Alternative name | MMP28 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Mmp28tm1Mani |
| Gene/Transgene symbol | Mmp28 |
Information from provider
| Provider | William C. Parks |
| Provider affiliation | Women, Cedars-Sinai Medical Center |
| Genetic information | The mouse Mmp28 gene was cloned from the 129/Sv genome (gene ID: 118453). To construct the homologous extensions of the targeting construct, a 4.8-kb EcoRI-XbaI fragment containing the 3 prime terminus of intron 1, exons 2 and 3, and 4 kb of intron 3 and a 2.1-kb XhoI-EcoRI fragment containing the 3 prime untranslated end of exon 8 and downstream sequence was isolated. A pgk-neomycin resistance cassette was inserted between the two arms and a diphtheria toxin (dta) cassette was ligated to the 3 prime end. The targeting construct was linearized with XhoI and introduced into RW-4 ES cells. |
| Phenotypic information | Homozygous:Homozygous MMP28-deficient mice have no overt phenotype. However, using a murine model of Pseudomonas aeruginosa pneumonia, it was found that Mmp28-/- mice had an early increase in macrophage recruitment into the lungs, as well as enhanced bacterial clearance and reduced pulmonary neutrophilia due to accelerated macrophage influx.Heterozygous:Heterozygous mice do not show an obvious phenotype. |
| Breeding history | 10 backcrosses to C57BL/6N. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institute of Molecular Genetics, Prague, Czech Republic |
| Animals used for archiving | homozygous C57BL/6NCrl males |
| Breeding at archiving centre | Proven breeder males were directly taken for sperm freezing. Males were homozygous for Mmp28 gene deletion. |
Disease and phenotype information
MGI phenotypes (gene matching)
Literature references
- Epilysin (MMP-28) restrains early macrophage recruitment in Pseudomonas aeruginosa pneumonia.;Manicone Anne M, Birkland Timothy P, Lin Michelle, Betsuyaku Tomoko, van Rooijen Nico, Lohi Jouko, Keski-Oja Jorma, Wang Ying, Skerrett Shawn J, Parks William C, ;2009;Journal of immunology (Baltimore, Md. : 1950);182;3866-76; 19265166