B6;129S-Cert1^tm1Jsau/Cnbc

Status	Available to order
EMMA ID	EM:08304
Citation information	RRID:IMSR_EM:08304 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6;129S-Cert1^tm1Jsau/Cnbc
Alternative name	Kin-LoxPe11-GPBP-1
Strain type	Targeted Mutant Strains : Conditional mutation
Allele/Transgene symbol	Cert1^tm1Jsau
Gene/Transgene symbol	Cert1

Information from provider

Provider	Juan Bautista Saus Mas
Provider affiliation	Bioquímica y Biología Molecular., Universidad de Valencia. Facultad de Medicina.
Genetic information	Targeted insertion of a loxP site between exons 10 and 11 as well as a floxed neomycin resistance cassette between exons 11 and 12. The loxP site between exons 10 and 11 was inserted using a PCR strategy.
Phenotypic information	Homozygous: There is no evident phenotype in this strain. Heterozygous: There is no evident phenotype in this strain.
Breeding history	To generate floxed Cert1 (GPBP-1) mice, previously obtained (neo+/loxP+) 129/SvEv ES cells transfected with a suitable replacement vector, were injected into C57BL/6 blastocysts. The resulting chimeric mice were further crossed to obtain homozygous (neo+/loxP+) mice. Later they were backcrossed to C57BL/6 and maintained during several years. There is no specific information about the number of generations backcrossed and sib-mated.
References	Goodpasture antigen-binding protein (GPBP) directs myofibril formation: identification of intracellular downstream effector 130-kDa GPBP-interacting protein (GIP130).;Revert-Ros Francisco, López-Pascual Ernesto, Granero-Moltó Froilán, Macías Jesús, Breyer Richard, Zent Roy, Hudson Billy G, Saadeddin Anas, Revert Fernando, Blasco Raül, Navarro Carmen, Burks Deborah, Saus Juan, ;2011;The Journal of biological chemistry;286;35030-43; 21832087
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	yes
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	homozygous unknown males, homozygous unknown females
Stage of embryos	2-cell

Disease and phenotype information

IMPC phenotypes (gene matching)

preweaning lethality, incomplete penetrance / IMPC
decreased mean corpuscular hemoglobin / IMPC

MGI phenotypes (gene matching)

abnormal heart morphology / MGI
thin ventricular wall / MGI
double outlet right ventricle / MGI
decreased cell proliferation / MGI
abnormal muscle development / MGI
abnormal forebrain morphology / MGI
abnormal cardiovascular system physiology / MGI
decreased embryo size / MGI
abnormal lipid homeostasis / MGI
abnormal nasal cavity morphology / MGI
abnormal semicircular canal morphology / MGI
persistent truncus arteriosis / MGI
absent tibia / MGI
no phenotypic analysis / MGI
aphakia / MGI
fetal growth retardation / MGI
abnormal optic cup morphology / MGI
abnormal vertebral arch morphology / MGI
fusion of vertebral bodies / MGI
fusion of vertebral arches / MGI
decreased cervical vertebrae number / MGI
homeostasis/metabolism phenotype / MGI
cellular phenotype / MGI
reproductive system phenotype / MGI
decreased ventricle muscle contractility / MGI
abnormal cell physiology / MGI
abnormal mitochondrion morphology / MGI
abnormal mitochondrial physiology / MGI
abnormal cardiac outflow tract development / MGI
increased heart ventricle size / MGI
abnormal cervical rib / MGI
abnormal endoplasmic reticulum morphology / MGI
abnormal cardiac jelly morphology / MGI
perimembraneous ventricular septal defect / MGI
muscular ventricular septal defect / MGI
abnormal pectinate muscle morphology / MGI
persistent right dorsal aorta / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
abnormal umbilical vein topology / MGI
abnormal vertebral artery topology / MGI
heterochrony / MGI
persistent trigeminal artery / MGI

Literature references

Goodpasture antigen-binding protein (GPBP) directs myofibril formation: identification of intracellular downstream effector 130-kDa GPBP-interacting protein (GIP130).;Revert-Ros Francisco, López-Pascual Ernesto, Granero-Moltó Froilán, Macías Jesús, Breyer Richard, Zent Roy, Hudson Billy G, Saadeddin Anas, Revert Fernando, Blasco Raül, Navarro Carmen, Burks Deborah, Saus Juan, ;2011;The Journal of biological chemistry;286;35030-43; 21832087

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

Right strain for your research?

The information provided on this page is, to the best of EMMA’s knowledge, based on data supplied by the original provider. End users are responsible for reviewing these details and for validating the strain and its suitability for their experimental use.
Not found what you were looking for? Search here for other strains available from EMMA.

B6;129S-Cert1tm1Jsau/Cnbc