B6.129S-Flrt2tm1Rob/RobH

Status

Available to order

EMMA IDEM:08321
Citation informationRRID:IMSR_EM:08321 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameB6.129S-Flrt2tm1Rob/RobH
Alternative nameFlrt2 tm1Rob
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolFlrt2tm1Rob
Gene/Transgene symbolFlrt2

Information from provider

ProviderElizabeth Robertson
Provider affiliationSir William Dunn School of Pathology, University of Oxford
Genetic informationThe 2.9 kb Flrt2 coding exon was replaced with a loxP-flanked PGK-hygromycin resistance cassette. This generated a null allele as assessed by lack of Flrt2 protein.
Phenotypic informationHomozygous:
Mouse embryos deficient in Flrt2 display defects in heart morphogenesis. In particular, defects in the integrity of the epicardium and disturbances to the architecture of the underlying basement membrane, accompanied by a pronounced reduction in thickness of the ventricular myocardium. A small proportion of homozygous mutants survives gestation (less than 3%).

Heterozygous:
Heterozygous mice have no phenotype.
Breeding historyStrain is currently maintained on C57BL/6J as heterozygous crosses.
References
  • The fibronectin leucine-rich repeat transmembrane protein Flrt2 is required in the epicardium to promote heart morphogenesis.;Müller Pari-Sima, Schulz Ramona, Maretto Silvia, Costello Ita, Srinivas Shankar, Bikoff Elizabeth, Robertson Elizabeth, ;2011;Development (Cambridge, England);138;1297-308; 21350012
Homozygous fertileno
Homozygous viableno
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

MGI phenotypes (allele matching)
  • pericardial edema / MGI
  • hemorrhage / MGI
  • abnormal epicardium morphology / MGI
  • abnormal endocardium morphology / MGI
  • embryo phenotype / MGI
  • ventricular myocardium compact layer hypoplasia / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • embryonic lethality during organogenesis, incomplete penetrance / MGI
  • lethality throughout fetal growth and development, incomplete penetrance / MGI
  • preweaning lethality, incomplete penetrance / MGI
MGI phenotypes (gene matching)
  • pericardial edema / MGI
  • hemorrhage / MGI
  • abnormal epicardium morphology / MGI
  • nervous system phenotype / MGI
  • abnormal endocardium morphology / MGI
  • embryo phenotype / MGI
  • abnormal neuronal migration / MGI
  • ventricular myocardium compact layer hypoplasia / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • embryonic lethality during organogenesis, incomplete penetrance / MGI
  • lethality throughout fetal growth and development, incomplete penetrance / MGI
  • preweaning lethality, incomplete penetrance / MGI

Literature references

  • The fibronectin leucine-rich repeat transmembrane protein Flrt2 is required in the epicardium to promote heart morphogenesis.;Müller Pari-Sima, Schulz Ramona, Maretto Silvia, Costello Ita, Srinivas Shankar, Bikoff Elizabeth, Robertson Elizabeth, ;2011;Development (Cambridge, England);138;1297-308; 21350012

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

Right strain for your research?

The information provided on this page is, to the best of EMMA’s knowledge, based on data supplied by the original provider. End users are responsible for reviewing these details and for validating the strain and its suitability for their experimental use.​
Not found what you were looking for? Search here for other strains available from EMMA.


Search
INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).