B6.129-Prdm4tm2.1Liz/RobH

Status

Available to order

EMMA IDEM:08322
Citation informationRRID:IMSR_EM:08322 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameB6.129-Prdm4tm2.1Liz/RobH
Alternative namePrdm4
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolPrdm4tm2.1Liz
Gene/Transgene symbolPrdm4

Information from provider

ProviderElizabeth Robertson
Provider affiliationSir William Dunn School of Pathology, University of Oxford
Genetic informationA targeted deletion of a 3.25 kb fragment encompassing exons 9, 10, and 11 that encodes 67 C-terminal amino acids of the PR/SET domain, the pseudo Zinc finger at position 548-569, and 5 proximal Zinc fingers within the ZFD (amino acid positions 593-699). This generated a functionally null allele as it resulted in a truncated Prdm4 protein which was predominantly cytoplasmic and failed to bind DNA.
Phenotypic informationHomozygous:
Mice with targeted deletion of the Zinc finger domain have no phenotype.

Heterozygous:
Heterozygous animals have no phenotype.
Breeding historyStrain is currently maintained on C57BL/6J as heterozygous crosses.
References
  • The PR/SET domain zinc finger protein Prdm4 regulates gene expression in embryonic stem cells but plays a nonessential role in the developing mouse embryo.;Bogani Debora, Morgan Marc A J, Nelson Andrew C, Costello Ita, McGouran Joanna F, Kessler Benedikt M, Robertson Elizabeth J, Bikoff Elizabeth K, ;2013;Molecular and cellular biology;33;3936-50; 23918801
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)
  • short tibia / IMPC
  • decreased total body fat amount / IMPC
  • increased cardiac muscle contractility / IMPC
  • abnormal cornea morphology / IMPC
  • microphthalmia / IMPC
  • impaired glucose tolerance / IMPC
  • abnormal heart left ventricle morphology / IMPC
  • enlarged urinary bladder / IMPC
  • absent spleen / IMPC
  • abnormal iris morphology / IMPC
  • increased lean body mass / IMPC
  • abnormal gait / IMPC
  • increased circulating amylase level / IMPC
  • thick ventricular wall / IMPC
MGI phenotypes (gene matching)
  • no abnormal phenotype detected / MGI
  • no phenotypic analysis / MGI

Literature references

  • The PR/SET domain zinc finger protein Prdm4 regulates gene expression in embryonic stem cells but plays a nonessential role in the developing mouse embryo.;Bogani Debora, Morgan Marc A J, Nelson Andrew C, Costello Ita, McGouran Joanna F, Kessler Benedikt M, Robertson Elizabeth J, Bikoff Elizabeth K, ;2013;Molecular and cellular biology;33;3936-50; 23918801

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Example health report
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