| Status | Available to order |
| EMMA ID | EM:08348 |
| Citation information | RRID:IMSR_EM:08348 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129P2-Dppa4tm1.1Hjf/Ieg |
| Alternative name | Dppa4-tdRFP (C57BL/6) |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Dppa4tm1.1Hjf |
| Gene/Transgene symbol | Dppa4 |
| Provider | Hans Joerg Fehling |
| Provider affiliation | University Clinics Ulm, Institute for Immunology |
| Genetic information | A "tandem-dimer red fluorescent protein" (tdRFP) expression cassette was inserted into exon 2 of the Dppa4 gene by homologous recombination to inactivate Dppa4 and at the same time to allow visualization of its expression based on tdRFP detection, e.g. by cytofluorometry or fluorescence microscopy. |
| Phenotypic information | Homozygous:Partial late embryonic/peri-natal lethality; deceased animals present with skeletal and lung defects; the few surviving animals appear phenotypically normal, but have reduced fertility. All pluripotent cells (cells in pre-implantation embryos, primordial germ cells and germ cells in adult mice) exhibit red fluorescence due to Dppa4-controlled expression of knock-in tdRFP cassetteHeterozygous:No apparent defects. All pluripotent cells (cells in pre-implantation embryos, primordial germ cells and germ cells in adult mice) exhibit red fluorescence due to Dppa4-controlled expression of knock-in tdRFP cassette |
| Breeding history | The knock-in/knock-out was generated in E14.1 ES cells (129/Ola). Chimeric mice were intercrossed with C57BL/6 partners. Heterozygous offspring carrying the modified allele was backcrossed for 20 generations onto C57BL/6. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | heterozygous C57BL/6 males, wild-type C57BL/6J females |
| Stage of embryos | 2-cell |
