STOCK Tg(Tnf*)A86Gkl/Flmg

Status	Available to order
EMMA ID	EM:08366
Citation information	RRID:IMSR_EM:08366 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	STOCK Tg(Tnf*)A86Gkl/Flmg
Alternative name	CBA.B6-Tg(Tnf*)A86Gkl/Flmg
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Tnf*)A86Gkl
Gene/Transgene symbol	Tg(Tnf*)A86Gkl

Information from provider

Provider	George Kollias
Provider affiliation	B.S.R.C.
Genetic information	This transgenic line expresses a mouse Tnf gene containing a deletion mutation of the codons encoding the 1st to the 12th amino acids of the mature 17-kDa TNF protein. This mutant TNF gene has previously been shown to produce a transmembrane TNF protein that remains bioactive against cellular targets in vitro and in vivo. Approximately 50 copies of the transgene were inserted. Northern analysis demonstrated transgene expression in thymus, mesenteric lymph nodes, spleen, kidney, liver, heart, brain, skin and joints. The expressed protein was undetectable in serum as determined by ELISA assays.
Phenotypic information	Homozygous: N/A Heterozygous: The mice develop chronic inflammatory arthritis, with 100% phenotypic penetrance, starting at 3-4 weeks of age. The arthritic phenotype is TNFRI-dependent and it is significantly delayed in the absence of the p75TNFR.
References	A murine transmembrane tumor necrosis factor (TNF) transgene induces arthritis by cooperative p55/p75 TNF receptor signaling.;Alexopoulou L, Pasparakis M, Kollias G, ;1997;European journal of immunology;27;2588-92; 9368614
Homozygous fertile	no
Homozygous viable	no
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	B.S.R.C. Alexander Fleming, Vari, Greece
Animals used for archiving	heterozygous CBA x C57BL/6 males

Literature references

A murine transmembrane tumor necrosis factor (TNF) transgene induces arthritis by cooperative p55/p75 TNF receptor signaling.;Alexopoulou L, Pasparakis M, Kollias G, ;1997;European journal of immunology;27;2588-92; 9368614

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Right strain for your research?

The information provided on this page is, to the best of EMMA’s knowledge, based on data supplied by the original provider. End users are responsible for reviewing these details and for validating the strain and its suitability for their experimental use.
Not found what you were looking for? Search here for other strains available from EMMA.