STOCK Cacna2d1^tm1Aschw/AschwAdlpnH

Status	Available to order
EMMA ID	EM:08421
Citation information	RRID:IMSR_EM:08421 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	STOCK Cacna2d1^tm1Aschw/AschwAdlpnH
Alternative name	C57BL6N
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Cacna2d1^tm1Aschw
Gene/Transgene symbol	Cacna2d1

Information from provider

Provider	Arnold Schwartz
Provider affiliation	Internal Medicine, University of Cincinnati
Genetic information	Cacna2d1 knockout.
Phenotypic information	Homozygous: In these mice, exon 2 of the targeted calcium channel 21 (Cacna2d1) allele is replaced with a neomycin resistance (neo) cassette abolishing Cacna2d1 gene function. Mice homozygous for this mutation exhibit a smooth muscle deficiency and increased urination. Homozygous males are impotent. Cacna2d1 KO mice lack gabapentin (GPB) binding sites, show decreased basal myocardial contractility and relaxation, and decreased L-type calcium currents in cardiomyocytes. Homozygous males have reduced mechanosensory responses and delayed development of neuropathic mechanical hypersensitivity following sensory nerve injury. Heterozygous: none detected
Breeding history	Mice were obtained from Prof. A. Schwartz, rederived at Harwell MGU and backcrossed twice by us. Number of generations backcrossed is unknown, but the mice were black coated when we obtained them.
References	Targeted disruption of the voltage-dependent calcium channel alpha2/delta-1-subunit.;Fuller-Bicer Geraldine A, Varadi Gyula, Koch Sheryl E, Ishii Masakazu, Bodi Ilona, Kadeer Nijiat, Muth James N, Mikala Gabor, Petrashevskaya Natalia N, Jordan Michael A, Zhang Sui-Po, Qin Ning, Flores Christopher M, Isaacsohn Idit, Varadi Maria, Mori Yasuo, Jones W Keith, Schwartz Arnold, ;2009;American journal of physiology. Heart and circulatory physiology;297;H117-24; 19429829 α2δ-1 gene deletion affects somatosensory neuron function and delays mechanical hypersensitivity in response to peripheral nerve damage.;Patel Ryan, Bauer Claudia S, Nieto-Rostro Manuela, Margas Wojciech, Ferron Laurent, Chaggar Kanchan, Crews Kasumi, Ramirez Juan D, Bennett David L H, Schwartz Arnold, Dickenson Anthony H, Dolphin Annette C, ;2013;The Journal of neuroscience : the official journal of the Society for Neuroscience;33;16412-26; 24133248
Homozygous fertile	females only
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Familial short QT syndrome / Orphanet_51083

IMPC phenotypes (gene matching)

impaired glucose tolerance / IMPC
decreased startle reflex / IMPC
decreased prepulse inhibition / IMPC
decreased exploration in new environment / IMPC
small spleen / IMPC
enhanced contextual conditioning behavior / IMPC
increased monocyte cell number / IMPC
abnormal freezing behavior / IMPC
increased circulating alkaline phosphatase level / IMPC
increased freezing behavior / IMPC
increased fasting circulating glucose level / IMPC
decreased bone mineral content / IMPC
decreased locomotor activity / IMPC
abnormal spleen morphology / IMPC

MGI phenotypes (allele matching)

distended urinary bladder / MGI
decreased channel response intensity / MGI
increased channel response threshold / MGI
abnormal muscle electrophysiology / MGI
decreased cardiac muscle contractility / MGI

MGI phenotypes (gene matching)

distended urinary bladder / MGI
abnormal CNS synaptic transmission / MGI
decreased channel response intensity / MGI
increased channel response threshold / MGI
abnormal muscle electrophysiology / MGI
decreased cardiac muscle contractility / MGI
impaired behavioral response to xenobiotic / MGI

Literature references

Targeted disruption of the voltage-dependent calcium channel alpha2/delta-1-subunit.;Fuller-Bicer Geraldine A, Varadi Gyula, Koch Sheryl E, Ishii Masakazu, Bodi Ilona, Kadeer Nijiat, Muth James N, Mikala Gabor, Petrashevskaya Natalia N, Jordan Michael A, Zhang Sui-Po, Qin Ning, Flores Christopher M, Isaacsohn Idit, Varadi Maria, Mori Yasuo, Jones W Keith, Schwartz Arnold, ;2009;American journal of physiology. Heart and circulatory physiology;297;H117-24; 19429829
α2δ-1 gene deletion affects somatosensory neuron function and delays mechanical hypersensitivity in response to peripheral nerve damage.;Patel Ryan, Bauer Claudia S, Nieto-Rostro Manuela, Margas Wojciech, Ferron Laurent, Chaggar Kanchan, Crews Kasumi, Ramirez Juan D, Bennett David L H, Schwartz Arnold, Dickenson Anthony H, Dolphin Annette C, ;2013;The Journal of neuroscience : the official journal of the Society for Neuroscience;33;16412-26; 24133248

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Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
Tissue - Types of tissue, service fee and delivery time available upon request

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STOCK Cacna2d1tm1Aschw/AschwAdlpnH