IMPC phenotypes (gene matching)
B6.Cg-Hnrnpdtm1.1Dkon/Flmg
| Status | Available to order |
| EMMA ID | EM:08426 |
| Citation information | RRID:IMSR_EM:08426 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Hnrnpdtm1.1Dkon/Flmg |
| Alternative name | B6.Cg-Hnrnpdtm1.1Dkon/Flmg |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Hnrnpdtm1.1Dkon |
| Gene/Transgene symbol | Hnrnpd |
Information from provider
| Provider | DIMITRIS KONTOYIANNIS |
| Provider affiliation | Institute of Immunology, Biomedical Sciences Research Centre (BSRC) "Alexander Fleming" |
| Genetic information | The construct was created with a BAC vector modified by VelociGene technology (Regeneron). It consisted of a neo cassette flanked by two FLPe recombination sites and two loxP sites. In the floxed allele FLPe recombination resulted in excision of the neo cassette. Upon cre-mediated recombination exons 3 and 4 are removed resulting in the expression of a mutated form of the HNRNPD (AUF1) protein that lacks the two RNA recognition motifs (RRMs), prohibiting RNA recognition. |
| Phenotypic information | Homozygous:No overt pathologyHeterozygous:No overt pathology |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | B.S.R.C. Alexander Fleming, Vari, Greece |
| Animals used for archiving | heterozygous C57BL/6J males, wild-type C57BL/6J females |
| Stage of embryos | 2-cell |
Disease and phenotype information
MGI phenotypes (gene matching)
- kidney hemorrhage / MGI
- decreased body weight / MGI
- decreased body size / MGI
- increased susceptibility to bacterial infection / MGI
- abnormal macrophage physiology / MGI
- fetal growth retardation / MGI
- increased circulating aspartate transaminase level / MGI
- increased blood urea nitrogen level / MGI
- increased circulating tumor necrosis factor level / MGI
- increased susceptibility to endotoxin shock / MGI
- decreased birth weight / MGI
- decreased birth body size / MGI
Literature references
- Inactivation of AUF1 in Myeloid Cells Protects From Allergic Airway and Tumor Infiltration and Impairs the Adenosine-Induced Polarization of Pro-Angiogenic Macrophages.;Gargani Sofia, Lourou Niki, Arapatzi Christina, Tzanos Dimitris, Saridaki Marania, Dushku Esmeralda, Chatzimike Margarita, Sidiropoulos Nikolaos D, Andreadou Margarita, Ntafis Vasileios, Hatzis Pantelis, Kostourou Vassiliki, Kontoyiannis Dimitris L, ;2022;Frontiers in immunology;13;752215; 35222366