IMPC phenotypes (gene matching)
B6.Cg-Hnrnpdtm1.2Dkon/Flmg
| Status | Available to order |
| EMMA ID | EM:08427 |
| Citation information | RRID:IMSR_EM:08427 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Hnrnpdtm1.2Dkon/Flmg |
| Alternative name | B6.Cg-Hnrnpdtm1.2Dkon/Flmg |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Hnrnpdtm1.2Dkon |
| Gene/Transgene symbol | Hnrnpd |
Information from provider
| Provider | Dimitris KONTOYIANNIS |
| Provider affiliation | Immunology, BSRC Al. Fleming |
| Genetic information | The construct was created with a BAC vector modified by VelociGene technology (Regeneron). It consisted of a neo cassette flanked by two FLPe recombination sites and two loxP sites. In the floxed allele FLPe recombination resulted in excision of the neo cassette; cre-mediated recombination removed the floxed exons 3 and 4, resulting in the expression of a mutated form of the HNRNPD (AUF1) protein that lacks the two RNA recognition motifs (RRMs), prohibiting RNA recognition. |
| Phenotypic information | Homozygous:Homozygous mutant mice for the Hnrnpdtm1.2Dkon allele display a sex-independent 50% divergence in Mendelian birth ratio and die before the end of gestation. Homozygous mice that are born are growth-retarded and sterile. Evaluation showed that homozygous mutant mice are almost half-sized in comparison to the wild-type mice.Heterozygous:No overt phenotype |
| References |
|
| Homozygous fertile | no |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | B.S.R.C. Alexander Fleming, Vari, Greece |
| Animals used for archiving | heterozygous C57BL/6J males |
Disease and phenotype information
MGI phenotypes (gene matching)
- kidney hemorrhage / MGI
- decreased body weight / MGI
- decreased body size / MGI
- increased susceptibility to bacterial infection / MGI
- abnormal macrophage physiology / MGI
- fetal growth retardation / MGI
- increased circulating aspartate transaminase level / MGI
- increased blood urea nitrogen level / MGI
- increased circulating tumor necrosis factor level / MGI
- increased susceptibility to endotoxin shock / MGI
- decreased birth weight / MGI
- decreased birth body size / MGI
Literature references
- Inactivation of AUF1 in Myeloid Cells Protects From Allergic Airway and Tumor Infiltration and Impairs the Adenosine-Induced Polarization of Pro-Angiogenic Macrophages.;Gargani Sofia, Lourou Niki, Arapatzi Christina, Tzanos Dimitris, Saridaki Marania, Dushku Esmeralda, Chatzimike Margarita, Sidiropoulos Nikolaos D, Andreadou Margarita, Ntafis Vasileios, Hatzis Pantelis, Kostourou Vassiliki, Kontoyiannis Dimitris L, ;2022;Frontiers in immunology;13;752215; 35222366