IMPC phenotypes (gene matching)
B6.129P2-Prdm5Gt(AV0702)Wtsi/Kctt
| Status | Available to order |
| EMMA ID | EM:08496 |
| Citation information | RRID:IMSR_EM:08496 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129P2-Prdm5Gt(AV0702)Wtsi/Kctt |
| Alternative name | Prdm5 LacZ |
| Strain type | Gene-trap |
| Allele/Transgene symbol | Prdm5Gt(AV0702)Wtsi |
| Gene/Transgene symbol | Prdm5 |
Information from provider
| Provider | Anders Lund |
| Provider affiliation | Pr. Anders Lund, Biotech Research and Innovation Centre, University of Copenhagen |
| Genetic information | To generate the Prdm5 LacZ strain, ES cell clone AV0702 from the Sanger Institute Gene Trap project was microinjected into C57BL/6 blastocysts. The integration site of the b-galactosidase-neomycin (b-geo) cassette in intron 2 of the Prdm5 gene was established. This cassette is preceded by a splice acceptor site to direct exon 2-b-geo splicing and ends with a poly-A site to terminate transcription. In mutant cells, expression of the Prdm5 locus results in the production of a fusion transcript between the first two exons of Prdm5 (less than 60 amino acids) and the b-geo cassette with a resulting fusion protein of approximately 135 kDa. The wt Prdm5 allele was quantified in Prdm5 LacZ/LacZ embryonic fibroblasts and found to reach a maximum of 10% relative to the expression in wt littermates, confirming the hypomorphic character of he strain. Prdm5 +/LacZ mice were backcrossed for at least 6 generations into the C57BL/6 strain. |
| Phenotypic information | Homozygous:Prdm5 is highly expressed in developing bones; and, by genome-wide mapping of Prdm5 occupancy in pre-osteoblastic cells. A novel and unique role for Prdm5 in targeting all mouse collagen genes as well as several small leucine rich proteoglycan (SLRP) genes was also found. In particular, Prdm5 controls both Collagen I transcription and fibrillogenesis by binding inside the Col1a1 gene body and maintaining RNA polymerase II occupancy. In vivo, Prdm5 loss results in delayed ossification involving a pronounced impairment in the assembly of fibrillar collagens. Therefore, a novel role for Prdm5 in sustaining the transcriptional program necessary to the proper assembly of osteoblastic extracellular matrix can be defined.Heterozygous:Not investigated |
| Breeding history | In parallel with intercross breedings of Prdm5 +/LacZ males to Prdm5 +/LacZ females to generate experimental cohorts, some Prdm5 +/LacZ males were backcrossed to C57BL/6 females to maintain the C57BL/6 purity of the strain. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Karolinska Institutet, Stockholm, Sweden |
| Animals used for archiving | heterozygous C57BL/6J males, wild-type females |
| Stage of embryos | 2-cell |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Brittle cornea syndrome / Orphanet_90354
MGI phenotypes (allele matching)
- delayed bone ossification / MGI
- decreased bone mineral density / MGI
- abnormal bone structure / MGI
- abnormal osteoblast morphology / MGI
- decreased bone mineral content / MGI
- decreased bone volume / MGI
- decreased trabecular bone volume / MGI
- decreased compact bone volume / MGI
- abnormal bone collagen fibril morphology / MGI
MGI phenotypes (gene matching)
- delayed bone ossification / MGI
- decreased bone mineral density / MGI
- abnormal bone structure / MGI
- abnormal osteoblast morphology / MGI
- decreased bone mineral content / MGI
- decreased bone volume / MGI
- decreased trabecular bone volume / MGI
- decreased compact bone volume / MGI
- abnormal bone collagen fibril morphology / MGI
Literature references
- Prdm5 regulates collagen gene transcription by association with RNA polymerase II in developing bone.;Galli Giorgio Giacomo, Honnens de Lichtenberg Kristian, Carrara Matteo, Hans Wolfgang, Wuelling Manuela, Mentz Bettina, Multhaupt Hinke Arnolda, Fog Cathrine Kolster, Jensen Klaus Thorleif, Rappsilber Juri, Vortkamp Andrea, Coulton Les, Fuchs Helmut, Gailus-Durner Valérie, Hrabě de Angelis Martin, Calogero Raffaele Adolfo, Couchman John Robert, Lund Anders Henrik, ;2012;PLoS genetics;8;e1002711; 22589746
- Genomic and proteomic analyses of Prdm5 reveal interactions with insulator binding proteins in embryonic stem cells.;Galli Giorgio Giacomo, Carrara Matteo, Francavilla Chiara, de Lichtenberg Kristian Honnens, Olsen Jesper Velgaard, Calogero Raffaele Adolfo, Lund Anders Henrik, ;2013;Molecular and cellular biology;33;4504-16; 24043305
- Prdm5 suppresses Apc(Min)-driven intestinal adenomas and regulates monoacylglycerol lipase expression.;Galli G G, Multhaupt H A, Carrara M, de Lichtenberg K H, Christensen I B J, Linnemann D, Santoni-Rugiu E, Calogero R A, Lund A H, ;2014;Oncogene;33;3342-50; 23873026