IMPC phenotypes (gene matching)
- hyperactivity / IMPC
C57BL/6N-Folh1em1Ph/Ph
| Status | Available to order |
| EMMA ID | EM:08499 |
| Citation information | RRID:IMSR_EM:08499 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6N-Folh1em1Ph/Ph |
| Alternative name | Folh1-em1Ph |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Folh1em1Ph |
| Gene/Transgene symbol | Folh1 |
Information from provider
| Provider | Jan Konvalinka |
| Provider affiliation | Dept. of Proteases of Human Pathogens, Institute of Organic Chemistry and Biochemie ASCR |
| Genetic information | The Folh1 (folate hydrolase 1) mutant mouse strain was prepared using TALEN technology. TALENs were designed to target exon 11 of murine Folh1 gene as follows: the left TALEN possessed the RVD sequence NG NG NN HD NI NI NN HD NG NN NN NN NI NG NN (targeted DNA sequence 5'-TTGCAAGCTGGGATG-3'), the right TALEN possessed the RVD sequence HD NI NN NG NI NN NI NI HD HD NI NI NN NI NI (targeted DNA sequence 5'-TTCTTGGTTCTACTG-3'). The TALENs were targeted to cleave within the chromosomal DNA sequence 5'-CAGAAGAATTTGGCC-3'. One founder mouse carrying deletion of 6 bp (5'-CAGAAGGCC-3') was chosen to establish this strain. The deletion lies in the region encoding the active site of the Folh1 protein. |
| Phenotypic information | Homozygous:Homozygous mice do not show any obvious phenotype. Homozygous mice appear normal.Heterozygous:Heterozygous mice do not show any obvious phenotype. Heterozygous mice appear normal. |
| Breeding history | One founder mouse was crossed to wild-type C57BL/6NCrl. Offspring from this was further bred to generate Folh1 homozygous mutant mice. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institute of Molecular Genetics, Prague, Czech Republic |
Disease and phenotype information
MGI phenotypes (gene matching)
- abnormal angiogenesis / MGI
- hippocampal neuron degeneration / MGI
- increased anxiety-related response / MGI
- abnormal sciatic nerve morphology / MGI
- increased systemic arterial blood pressure / MGI
- enhanced coordination / MGI
- abnormal peripheral nervous system regeneration / MGI
- decreased susceptibility to injury / MGI
- behavior/neurological phenotype / MGI
- decreased angiogenesis / MGI
- abnormal vascular endothelial cell morphology / MGI
- decreased cerebral infarction size / MGI
- decreased susceptibility to ischemic brain injury / MGI
- mortality/aging / MGI
- increased food intake / MGI
- decreased astrocyte number / MGI
- enhanced spatial learning / MGI