B6J.Cg-Tg(RAB27A-EGFP/Rab27a)84Seab/SeabH
| Status | Available to order |
| EMMA ID | EM:08532 |
| Citation information | RRID:IMSR_EM:08532 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6J.Cg-Tg(RAB27A-EGFP/Rab27a)84Seab/SeabH |
| Alternative name | EGFP-Rab27a |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(RAB27A-EGFP/Rab27a)84Seab |
| Gene/Transgene symbol | Tg(RAB27A-EGFP/Rab27a)84Seab |
Information from provider
| Provider | Miguel Seabra |
| Provider affiliation | Molecular Medicine Section, National Heart & Lung Institute, Imperial College London |
| Additional owner | Prof. Miguel Seabra and Dr. Tanya Tolmachova, Imperial College, London, UK |
| Genetic information | Transgene EGFP-Rab27a is a PAC-based transgene. The original, unmodified PAC was obtained from Genome Systems, St. Louis, MO (PAC #15798). The modified PAC contains Rab27a human gene. EGFP-Rab27a rat cDNA fusion gene was inserted into the PAC at the start codon, so that EGFP-Rab27a expression is driven by Rab27 promoter within the PAC. Expression of EGFP-Rab27a protein faithfully follows expression of endogenous Rab27a mouse gene as detected by Western blot. To assess functionality of the transgene EGFP-Rab27a transgenic mice were crossed with a Rab27a-mutant line (ashen, ash/ash). The Rab27a defect in ashen mice leads to coat colour dilution and defect in cytotoxic T lymphocytes (CTLs). In Rab27a ash/ash EGFP-Rab27a line both defects of the ashen Rab27a knockout mouse (coat colour dilution and CTL defect) were rescued, thus confirming transgene functionality (see Tolmachova et. al., Mol Biol Cell (2004) 15, 332-344). |
| Phenotypic information | Homozygous:Mutation is maintained as hemizygotes; homozygous animals were not generatedHeterozygous:Phenotype of hemizygous mice is normal, no abnormalities |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- A general role for Rab27a in secretory cells.;Tolmachova Tanya, Anders Ross, Stinchcombe Jane, Bossi Giovanna, Griffiths Gillian M, Huxley Clare, Seabra Miguel C, ;2004;Molecular biology of the cell;15;332-44; 14617806