BALB/c-Tg(CAG-Phlp5*,-EGFP)304Biat/Biat

Status	Available to order
EMMA ID	EM:08925
Citation information	RRID:IMSR_EM:08925 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	BALB/c-Tg(CAG-Phlp5*,-EGFP)304Biat/Biat
Alternative name	mPhl p 5 IRES GFP BALB/c
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(CAG-Phlp5*,-EGFP)304Biat
Gene/Transgene symbol	Tg(CAG-Phlp5*,-EGFP)304Biat

Information from provider

Provider	Thomas Wekerle
Provider affiliation	Department of Surgery, Division of Transplantation, Medical University of Vienna
Genetic information	The cDNA of Phl p 5, one major clinically relevant allergen of Timothy grass (Phleum pratense) was fused to a transmembrane domain and a leader peptide (described in Baranyi et al., J Immunol 2008, 180: 8168-8175) and cloned into pcall2 IRES-eGFP vector, under control of a chicken beta globulin promoter and CMV enhancer.
Phenotypic information	Mice express Phl p 5 on the surface of all cells e.g. hematopoietic cells and tested tissues (heart, skin, spleen, thymus) and coexpress GFP in the cytoplasm.
Breeding history	more than 10 generations
References	Cell Therapy for Prophylactic Tolerance in Immunoglobulin E-mediated Allergy.;Baranyi Ulrike, Farkas Andreas M, Hock Karin, Mahr Benedikt, Linhart Birgit, Gattringer Martina, Focke-Tejkl Margit, Petersen Arnd, Wrba Fritz, Rülicke Thomas, Valenta Rudolf, Wekerle Thomas, ;2016;EBioMedicine;7;230-9; 27322476
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	University of Veterinary Medicine, Vienna, Austria
Animals used for archiving	homozygous males

Literature references

Cell Therapy for Prophylactic Tolerance in Immunoglobulin E-mediated Allergy.;Baranyi Ulrike, Farkas Andreas M, Hock Karin, Mahr Benedikt, Linhart Birgit, Gattringer Martina, Focke-Tejkl Margit, Petersen Arnd, Wrba Fritz, Rülicke Thomas, Valenta Rudolf, Wekerle Thomas, ;2016;EBioMedicine;7;230-9; 27322476

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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