B6.129-Grin2a^tm2Rsp/Kctt

Status	Available to order
EMMA ID	EM:09319
Citation information	RRID:IMSR_EM:09319 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.129-Grin2a^tm2Rsp/Kctt
Alternative name	Grin2A(N615S) (lab name N2AMS)
Strain type	Targeted Mutant Strains : Knock-in
Allele/Transgene symbol	Grin2a^tm2Rsp
Gene/Transgene symbol	Grin2a

Information from provider

Provider	Rolf Sprengel
Provider affiliation	Molecular Neurobiology, Max Planck Institute for Medical Research
Genetic information	In the gene for the NMDA receptor subunit GluN2A (Grin2a) a single amino acid codon that determines the voltage dependence of the NMDA receptor was replaced by gene targeting. In the mutated GluN2A subunit the amino acid Asparagine is replaced by Serine. In vitro studies with recombinant receptors showed that the gating of the mutated NMDA receptor is voltage independent.
Phenotypic information	Homozygous: In homozygous mice audiogenic seizures can be induced with a 100% efficiency. Homozygous mice show many learning and memory impairments. Heterozygous: Some heterozygous mice show audiogenic induced seizure impairments. No significant learning impairments could be detected up to now.
Breeding history	In August 2014, 32 embryos of C57BL/6 and heterozygous Grin2a(N615S)/+ mice were harvested and cryoconserved.
References	Gene targeted mice with the NMDA receptor subunit NR2A point mutation N596S show elevated levels of LTP;Single FN, Rozov A, Burnashev N, Wollmuth LP, Kuner T, Jensen V, Hvalny O, Sprengel R, Seeburg PH;2002;Annual Meeting of the Society for Neuroscience in Orlando 2002 ;4;Poster 150.6 ; 33420383 Voltage-independent GluN2A-type NMDA receptor Ca²⁺ signaling promotes audiogenic seizures, attentional and cognitive deficits in mice.;Bertocchi Ilaria, Eltokhi Ahmed, Rozov Andrey, Chi Vivan Nguyễn, Jensen Vidar, Bus Thorsten, Pawlak Verena, Serafino Marta, Sonntag Hannah, Yang Boyi, Burnashev Nail, Li Shi-Bin, Obenhaus Horst A, Both Martin, Niewoehner Burkhard, Single Frank N, Briese Michael, Boerner Thomas, Gass Peter, Rawlins John Nick P, Köhr Georg, Bannerman David M, Sprengel Rolf, ;2021;Communications biology;181;59; 38529699 Radiprodil, a selective GluN2B negative allosteric modulator, rescues audiogenic seizures in mice carrying the GluN2A(N615S) mutation.;Bertocchi Ilaria, Cifarelli Lorenzo, Oberto Alessandra, Eva Carola Eugenia, Sprengel Rolf, Mirza Naheed Rohman, Muglia Pierandrea, ;2024;British journal of pharmacology;;1886-1894;
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	not known

Information from EMMA

Archiving centre	Karolinska Institutet, Stockholm, Sweden
Animals used for archiving	heterozygous C57BL/6 males, wild-type C57BL/6N females
Stage of embryos	2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Landau-Kleffner syndrome / Orphanet_98818
Continuous spikes and waves during sleep / Orphanet_725
Rolandic epilepsy-speech dyspraxia syndrome / Orphanet_163721
Rolandic epilepsy / Orphanet_1945
Early-onset epileptic encephalopathy and intellectual disability due to GRIN2A mutation / Orphanet_289266

MGI phenotypes (gene matching)

decreased anxiety-related response / MGI
jumpy / MGI
impaired coordination / MGI
decreased exploration in new environment / MGI
abnormal spatial learning / MGI
reduced long term potentiation / MGI
increased startle reflex / MGI
reduced kindling response / MGI
reduced NMDA-mediated synaptic currents / MGI
decreased chemically-elicited antinociception / MGI
decreased vertical activity / MGI
abnormal excitatory postsynaptic currents / MGI
allodynia / MGI
abnormal depression-related behavior / MGI
nervous system phenotype / MGI
decreased thermal nociceptive threshold / MGI
abnormal miniature excitatory postsynaptic currents / MGI
behavior/neurological phenotype / MGI
abnormal spatial working memory / MGI
decreased chemical nociceptive threshold / MGI
impaired contextual conditioning behavior / MGI

Literature references

Gene targeted mice with the NMDA receptor subunit NR2A point mutation N596S show elevated levels of LTP;Single FN, Rozov A, Burnashev N, Wollmuth LP, Kuner T, Jensen V, Hvalny O, Sprengel R, Seeburg PH;2002;Annual Meeting of the Society for Neuroscience in Orlando 2002 ;4;Poster 150.6 ; 33420383
Voltage-independent GluN2A-type NMDA receptor Ca²⁺ signaling promotes audiogenic seizures, attentional and cognitive deficits in mice.;Bertocchi Ilaria, Eltokhi Ahmed, Rozov Andrey, Chi Vivan Nguyễn, Jensen Vidar, Bus Thorsten, Pawlak Verena, Serafino Marta, Sonntag Hannah, Yang Boyi, Burnashev Nail, Li Shi-Bin, Obenhaus Horst A, Both Martin, Niewoehner Burkhard, Single Frank N, Briese Michael, Boerner Thomas, Gass Peter, Rawlins John Nick P, Köhr Georg, Bannerman David M, Sprengel Rolf, ;2021;Communications biology;181;59; 38529699
Radiprodil, a selective GluN2B negative allosteric modulator, rescues audiogenic seizures in mice carrying the GluN2A(N615S) mutation.;Bertocchi Ilaria, Cifarelli Lorenzo, Oberto Alessandra, Eva Carola Eugenia, Sprengel Rolf, Mirza Naheed Rohman, Muglia Pierandrea, ;2024;British journal of pharmacology;;1886-1894;

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

Right strain for your research?

The information provided on this page is, to the best of EMMA’s knowledge, based on data supplied by the original provider. End users are responsible for reviewing these details and for validating the strain and its suitability for their experimental use.
Not found what you were looking for? Search here for other strains available from EMMA.

B6.129-Grin2atm2Rsp/Kctt