STOCK Tg(Ttr-NR0B2)1Ital/Flmg

Status	Available to order
EMMA ID	EM:09491
Citation information	RRID:IMSR_EM:09491 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	STOCK Tg(Ttr-NR0B2)1Ital/Flmg
Alternative name	B6.CBA-Tg(Ttr-NR0B2)1Ital/Flmg
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Ttr-NR0B2)1Ital
Gene/Transgene symbol	Tg(Ttr-NR0B2)1Ital

Information from provider

Provider	Iannis Talianidis
Provider affiliation	BSRC Alexander Fleming
Genetic information	The transgenic construct expresses human NR0B2 (former SHP) (HA-Tag) flagged under the mouse Ttr promoter. Hepatic SHP expression in this transgenic line is 7-fold above endogenous levels.
Phenotypic information	Homozygous: The livers of transgenic animals are visibly enlarged and pale in color. Extensive lipid accumulation is detected in SHP-expressing hepatocytes. Hepatic triglyceride levels are markedly increased and the concentration of hepatic bile acids is significantly decreased. Major defects in Tg-SHP mice occur in bile acid synthesis, lipogenesis, de novo cholesterol biosynthesis and absorption pathways. Heterozygous: NOT EXAMINED
References	Regulation of hepatic metabolic pathways by the orphan nuclear receptor SHP.;Boulias Konstantinos, Katrakili Nitsa, Bamberg Krister, Underhill Peter, Greenfield Andy, Talianidis Iannis, ;2005;The EMBO journal;24;2624-33; 15973435
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	B.S.R.C. Alexander Fleming, Vari, Greece
Animals used for archiving	heterozygous C57BL/6.CBA males, wild-type (C57BL/6 x CBA)F1 females
Stage of embryos	2-cell

Literature references

Regulation of hepatic metabolic pathways by the orphan nuclear receptor SHP.;Boulias Konstantinos, Katrakili Nitsa, Bamberg Krister, Underhill Peter, Greenfield Andy, Talianidis Iannis, ;2005;The EMBO journal;24;2624-33; 15973435

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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