B6;129S6-Sp140tm1Ptsn/Kctt
| Status | Available to order |
| EMMA ID | EM:09496 |
| Citation information | RRID:IMSR_EM:09496 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;129S6-Sp140tm1Ptsn/Kctt |
| Alternative name | B6;129S6-Sp140-/- |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Sp140tm1Ptsn |
| Gene/Transgene symbol | Sp140 |
Information from provider
| Provider | Mario Saare |
| Provider affiliation | Molecular Pathology group, Institute of Biomedicine and Translational Medicine, University of Tartu |
| Genetic information | The ES cells derived from 129S6/SvEvTac mouse strain were electroporated with a vector containing the neo and TK cassettes for selection, and loxP1 sites that were used to cut out the neo cassette together with exon 9 and partially exon 10. The cloned ES cells were transferred to C57BL/6J blastocysts. |
| Phenotypic information | Sp140 is strongly expressed in B cells. We anticipated the knock-out to have an effect on the levels of different immunoglobulin classes, but the antibody production was unaltered. Also, we tested the avidity of ovalbumin-specific antibodies, but no changes could be determined. B cell population numbers and proportions were normal. The animals are fertile and age normally. |
| Breeding history | The strain has not been backcrossed. The mice have been inbred for five generations. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Karolinska Institutet, Stockholm, Sweden |
| Animals used for archiving | heterozygous C57BL/6J males |