B6;CBA-Tg(CAG-GFPuv/AEQ*)30Alns/Cnbc
| Status | Available to order |
| EMMA ID | EM:09599 |
| Citation information | RRID:IMSR_EM:09599 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;CBA-Tg(CAG-GFPuv/AEQ*)30Alns/Cnbc |
| Alternative name | erGAP1 L30 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(CAG-GFPuv/AEQ*)30Alns |
| Gene/Transgene symbol | Tg(CAG-GFPuv/AEQ*)30Alns |
Information from provider
| Provider | Maria Teresa Alonso |
| Provider affiliation | IBGM-Universidad de Valladolid |
| Genetic information | The gene erGAP1 comprises the calreticulin signal peptide and the sequence encoding the ER retention KDEL fused in frame to the 5'- and 3'-end of GAP1 (GFP-Aequorin Protein 1), respectively. Genetically encoded calcium indicators (GECIs) are excellent tools to monitor subcellular Ca2+ signals inside organelles. However, most GECIs are fusions of endogenous calcium-binding proteins whose functionality in vivo may be perturbed by competition with cellular partners. We describe here a novel family of fluorescent Ca2+ sensors based on the fusion of two jellyfish proteins, GFP and aequorin, which should minimally interfere with endogenous functions. The new biosensor displays many advantages over other existing Ca2+ probes, including its ability to be targeted to multiple organelles and its functionality in vivo. The new biosensor fills a gap in the actual repertoire of Ca2+ indicators for organelles and becomes a valuable tool for in vivo Ca2+ imaging applications. |
| Phenotypic information | These transgenic mice express a new fluorescent calcium indicator based on the fusion of GFP and aequorin (GAP) into the endoplasmic reticulum. It becomes a valuable tool for in vivo Ca2+ imaging applications, specially in neuronal tissues. |
| Breeding history | Since 7 generations brother x sister matings have been performed. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous C57BL/6 males, wild-type females |
| Stage of embryos | 2-cell |
Literature references
- GAP, an aequorin-based fluorescent indicator for imaging Ca2+ in organelles.;Rodriguez-Garcia Arancha, Rojo-Ruiz Jonathan, Navas-Navarro Paloma, Aulestia Francisco Javier, Gallego-Sandin Sonia, Garcia-Sancho Javier, Alonso Maria Teresa, ;2014;Proceedings of the National Academy of Sciences of the United States of America;111;2584-9; 24501126