MGI phenotypes (allele matching)
B6;129X1-Decr1tm1Jkh/Oulu[cc]
| Status | Available to order |
| EMMA ID | EM:09657 |
| Citation information | RRID:IMSR_EM:09657 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;129X1-Decr1tm1Jkh/Oulu[cc] |
| Alternative name | Decr-knockout (in C57BL/6) |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Decr1tm1Jkh |
| Gene/Transgene symbol | Decr1 |
Information from provider
| Provider | Ilkka Miinalainen |
| Provider affiliation | Biocenter Oulu |
| Genetic information | The genomic clone BACM:109-18E (from 129/SvJ strain) corresponding to the mouse Decr locus was obtained from Genome Systems (St Louis, MO, USA). A 3.3 kb EcoRI–HindIII fragment upstream of the first exon was cloned into a Bluescript vector modified with SalI and ClaI sites flanking the polylinker. A 4.4 kb SmaI–EcoRV fragment from the first intron was cloned into a Bluescript vector modified with AscI and PacI sites flanking the polylinker. For the replacement vector, SalI–ClaI and AscI–PacI cleaved fragments were ligated to the corresponding sites of the pPGKneo/TK-2 vector, where they flanked the PGKneo cassette. The neomycin resistance (neo) and thymidine kinase (TK) genes were used for positive and negative selection, respectively. Linearized replacement vector was electroporated into RW-4 embryonic stem (ES) cells (129/SvJ, Tyrchp/Tyrcp) that were subsequently grown under G418 and ganciclovir selection. Correctly targeted ES cell clones were identified by Southern analysis of genomic DNA, for which the BamHI restriction fragment length polymorphism created by homologous integration was identified by a 59-probe upstream of the targeted locus. Germline chimeric mice were produced by microinjecting ES cells from positive clones into C57BL/6 blastocysts at the Biocenter Oulu Transgenic core facility. |
| Phenotypic information | Homozygous:Under standard laboratory conditions, Decr-/- mice were indistinguishable from wild-type mice. Crossbreeding of Decr +/- mice produced progeny in approximately Mendelian ratios, with no gender bias. Both male and female mutant mice were viable and fertile. They exhibited weight gain and life-span similar to that of wild-type mice. Analysis of organ weights and histological analysis of major organs, including liver, muscle, heart, kidney, lungs, spleen and intestine, showed no differences between wild-type and mutant mice.Heterozygous:No observed phenotype |
| Breeding history | Sib-mating has been avoided whenever possible, maintenance in +/- genotypes, backcrossing to wild-type approximately once a year. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Oulu, Oulu, Finland |
| Animals used for archiving | homozygous males |
Disease and phenotype information
Literature references
- Mitochondrial 2,4-dienoyl-CoA reductase deficiency in mice results in severe hypoglycemia with stress intolerance and unimpaired ketogenesis.;Miinalainen Ilkka J, Schmitz Werner, Huotari Anne, Autio Kaija J, Soininen Raija, Ver Loren van Themaat Emiel, Baes Myriam, Herzig Karl-Heinz, Conzelmann Ernst, Hiltunen J Kalervo, ;2009;PLoS genetics;5;e1000543; 19578400