B6.129-Msx2tm1Bero/Kctt

Status

Available to order

EMMA IDEM:09720
Citation informationRRID:IMSR_EM:09720 

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International strain nameB6.129-Msx2tm1Bero/Kctt
Alternative nameMsx2tm1Bero (Synonym : Msx2-nlacZ)
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolMsx2tm1Bero
Gene/Transgene symbolMsx2

Information from provider

ProviderBenoit Robert
Provider affiliationDevelopmental and Stem Cell Biology, Institut Pasteur
Genetic informationThe complete nlacZ coding sequence has been inserted in frame at the ATG start codon of the Msx2 locus. The resulting allele is a null allele for Msx2, expressing a nuclear form of the beta-galactosidase enzyme instead of the genuine Msx2 protein.
Phenotypic informationHomozygous:
Msx2tm1Bero mutation (Synonym: Msx2-nlacZ) is a null Msx2 mutation; homozygotes exhibit pleiotropic defects of skeletal and ectodermal organs (abnormal calvaria development of the skull, defective chondrogenesis and endochondral ossification of long bones, hair-loss, mammary gland defects, molar tooth degeneration). In addition they also display foliation and lamination defects in the cerebellum.

Heterozygous:
The heterozygotes are normal.
Breeding history15 backcrosses to C57BL/6
References
  • Msx1 is required for dorsal diencephalon patterning.;Bach Antoine, Lallemand Yvan, Nicola Marie-Anne, Ramos Casto, Mathis Luc, Maufras Mathilde, Robert Benoît, ;2003;Development (Cambridge, England);130;4025-36; 12874124
Homozygous fertileno
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreKarolinska Institutet, Stockholm, Sweden
Animals used for archivingheterozygous C57BL/6J males

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (gene matching)
  • big ears / MGI
  • decreased bone mineral density / MGI
  • abnormal neurocranium morphology / MGI
  • abnormal frontal bone morphology / MGI
  • malocclusion / MGI
  • brittle teeth / MGI
  • degenerate molars / MGI
  • ameloblast degeneration / MGI
  • abnormal trabecular bone morphology / MGI
  • decreased compact bone thickness / MGI
  • abnormal long bone hypertrophic chondrocyte zone / MGI
  • decreased chondrocyte number / MGI
  • alopecia / MGI
  • delayed hair regrowth / MGI
  • abnormal nail morphology / MGI
  • deformed nails / MGI
  • long toenails / MGI
  • abnormal cerebellum development / MGI
  • cerebellum vermis hypoplasia / MGI
  • abnormal cerebellum anterior vermis morphology / MGI
  • absent cerebellum vermis lobule VIII / MGI
  • absent cerebellum vermis lobule IX / MGI
  • delaminated Purkinje cell layer / MGI
  • delaminated cerebellar granule layer / MGI
  • curly vibrissae / MGI
  • short vibrissae / MGI
  • abnormal eye development / MGI
  • abnormal lens vesicle development / MGI
  • microphthalmia / MGI
  • abnormal lens morphology / MGI
  • small lens / MGI
  • abnormal cornea morphology / MGI
  • corneal opacity / MGI
  • abnormal retina morphology / MGI
  • abnormal eye morphology / MGI
  • abnormal skeleton development / MGI
  • abnormal axial skeleton morphology / MGI
  • no abnormal phenotype detected / MGI
  • chondrodystrophy / MGI
  • short tibia / MGI
  • tonic seizures / MGI
  • short femur / MGI
  • abnormal optic vesicle formation / MGI
  • nervous system phenotype / MGI
  • embryonic growth retardation / MGI
  • abnormal mammary gland embryonic development / MGI
  • frontal bone foramen / MGI
  • small interparietal bone / MGI
  • small supraoccipital bone / MGI
  • decreased osteoclast cell number / MGI
  • premature hair loss / MGI
  • abnormal retinal pigment epithelium morphology / MGI
  • narrow eye opening / MGI
  • cardiovascular system phenotype / MGI
  • behavior/neurological phenotype / MGI
  • vitreous body deposition / MGI
  • abnormal bone ossification / MGI
  • abnormal hair shedding / MGI
  • abnormal appendicular skeleton morphology / MGI
  • increased corneal stroma thickness / MGI
  • abnormal enamel organ morphology / MGI
  • mortality/aging / MGI
  • integument phenotype / MGI
  • anterior iris synechia / MGI
  • increased cornea thickness / MGI
  • long nails / MGI
  • abnormal nail matrix morphology / MGI
  • iris hyperplasia / MGI
  • decreased corneal epithelium thickness / MGI
  • abnormal stellate reticulum morphology / MGI
  • abnormal stratum intermedium morphology / MGI

Literature references

  • Msx1 is required for dorsal diencephalon patterning.;Bach Antoine, Lallemand Yvan, Nicola Marie-Anne, Ramos Casto, Mathis Luc, Maufras Mathilde, Robert Benoît, ;2003;Development (Cambridge, England);130;4025-36; 12874124

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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