B6.Cg-Tg(Wap-Shh)119Mig/Cnbc

Status	Available to order
EMMA ID	EM:09854
Citation information	RRID:IMSR_EM:09854 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.Cg-Tg(Wap-Shh)119Mig/Cnbc
Alternative name	C57Bl6.Tg(Wap-Shh)119Mig
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Wap-Shh)119Mig
Gene/Transgene symbol	Tg(Wap-Shh)119Mig

Information from provider

Provider	Marta Gallego
Provider affiliation	Patología Mamaria, Instituto de Salud Carlos III
Genetic information	Transgenic Shh expression in the secretory epithelium of the mouse mammary gland under whey acidic protein (WAP) promoter in C57BL/6 genetic background.
Phenotypic information	Homozygous: Do not apply Heterozygous: Transgenic Shh expression in the secretory epithelium of the mouse mammary gland induces the expansion of ciliated cells that express diverse progenitor cell markers in the mammary basal epithelium. Mammary glands number 3 and 4 are fused on each side under the skin. Left or right number three nipples are missing in 20% of transgenic mice. C57BL/6-Tg(Wap-Shh)119Mig mice do not have the kinky tail phenotype that apppears in FVB.Cg-Tg(Wap-Shh)119Mig/Cnbc mice .
Breeding history	N=9 backcrosses to C57BL/6
References	Intraepithelial paracrine Hedgehog signaling induces the expansion of ciliated cells that express diverse progenitor cell markers in the basal epithelium of the mouse mammary gland.;García-Zaragoza Elena, Pérez-Tavarez Raquel, Ballester Alicia, Lafarga Vanesa, Jiménez-Reinoso Anaïs, Ramírez Angel, Murillas Rodolfo, Gallego Marta I, ;2012;Developmental biology;372;28-44; 23000969
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	heterozygous C57BL/6JOlaHsd males, wild-type C57BL/6JOlaHsd females
Stage of embryos	2-cell

Literature references

Intraepithelial paracrine Hedgehog signaling induces the expansion of ciliated cells that express diverse progenitor cell markers in the basal epithelium of the mouse mammary gland.;García-Zaragoza Elena, Pérez-Tavarez Raquel, Ballester Alicia, Lafarga Vanesa, Jiménez-Reinoso Anaïs, Ramírez Angel, Murillas Rodolfo, Gallego Marta I, ;2012;Developmental biology;372;28-44; 23000969

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
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Material Transfer Agreement (MTA)
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Legally binding conditions for the transfer

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