B6;129P2(Cg)-Dkk1^tm1.1Svo/Oulu

Status	Available to order
EMMA ID	EM:09872
Citation information	RRID:IMSR_EM:09872 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6;129P2(Cg)-Dkk1^tm1.1Svo/Oulu
Alternative name	Dkk1 (tm1.1Svo)
Strain type	Targeted Mutant Strains : Conditional mutation
Allele/Transgene symbol	Dkk1^tm1.1Svo
Gene/Transgene symbol	Dkk1

Information from provider

Provider	Christof Niehrs
Provider affiliation	Institute of Molecular Biology (IMB), University of Mainz
Genetic information	Targeted construct in 129/Ola ES cells modifies the Dkk1 allele so that Dkk1 exons 1 and 2 are flanked by loxP sites.
Phenotypic information	Homozygous: Homozygous Dkk1 flox/flox mice do not have any phenotypes as it requires cre recombinase activation to delete exons 1 and 2. Heterozygous: Heterozygous Dkk1 flox/+ mice do not have any phenotypes as it requires cre recombinase activation to delete exons 1 and 2.
Breeding history	Targeted mutation was directly maintained on a C57BL/6 background.
References	Secreted Wnt antagonist Dickkopf-1 controls kidney papilla development coordinated by Wnt-7b signalling.;Pietilä Ilkka, Ellwanger Kristina, Railo Antti, Jokela Tiina, Barrantes Ivan Del Barco, Shan Jingdong, Niehrs Christof, Vainio Seppo J, ;2011;Developmental biology;353;50-60; 21354128
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	University of Oulu, Oulu, Finland
Animals used for archiving	homozygous males

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Arnold-Chiari malformation type I / Orphanet_268882

MGI phenotypes (gene matching)

absent mandible / MGI
microcephaly / MGI
abnormal forelimb morphology / MGI
abnormal hindlimb morphology / MGI
polydactyly / MGI
syndactyly / MGI
ectopic digits / MGI
abnormal telencephalon morphology / MGI
anophthalmia / MGI
thick apical ectodermal ridge / MGI
abnormal craniofacial development / MGI
abnormal embryonic neuroepithelium morphology / MGI
small interparietal bone / MGI
small parietal bone / MGI
absent nasal bone / MGI
absent maxilla / MGI
abnormal limb bud morphology / MGI
absent nasal placodes / MGI
absent optic vesicle / MGI
abnormal first pharyngeal arch morphology / MGI
abnormal mandibular prominence morphology / MGI
neonatal lethality, complete penetrance / MGI
decreased midbrain size / MGI
decreased forebrain size / MGI
rostral body truncation / MGI
absent frontonasal prominence / MGI
absent diencephalon / MGI
abnormal anterior head development / MGI

Literature references

Secreted Wnt antagonist Dickkopf-1 controls kidney papilla development coordinated by Wnt-7b signalling.;Pietilä Ilkka, Ellwanger Kristina, Railo Antti, Jokela Tiina, Barrantes Ivan Del Barco, Shan Jingdong, Niehrs Christof, Vainio Seppo J, ;2011;Developmental biology;353;50-60; 21354128
Small-molecule Wnt agonists correct cleft palates in Pax9 mutant mice in utero.;Jia Shihai, Zhou Jing, Fanelli Christopher, Wee Yinshen, Bonds John, Schneider Pascal, Mues Gabriele, D'Souza Rena N, ;2017;Development (Cambridge, England);144;3819-3828; 28893947
Leishmania major surface components and DKK1 signalling via LRP6 promote migration and longevity of neutrophils in the infection site.;Ihedioha Olivia C, Marcarian Haley Q, Sivakoses Anutr, Beverley Stephen M, McMahon-Pratt Diane, Bothwell Alfred L M, ;2024;Frontiers in immunology;15;1473133; 39502693
Platelet DKK1 promotes tolerogenic dendritic cells and non-healing responses in cutaneous leishmaniasis.;Ihedioha Olivia C, Sivakoses Anutr, Marcarian Haley Q, Sajeev Malini, McMahon-Pratt Diane, Bothwell Alfred L M, ;2025;bioRxiv : the preprint server for biology;0;; 40502169

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6;129P2(Cg)-Dkk1tm1.1Svo/Oulu