B6.Cg-Tg(Col13a1)2Pih/Oulu
| Status | Available to order |
| EMMA ID | EM:09885 |
| Citation information | RRID:IMSR_EM:09885 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Tg(Col13a1)2Pih/Oulu |
| Alternative name | B6.Cg-Tg(Col13a1)2Pih/Oulu |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Col13a1)2Pih |
| Gene/Transgene symbol | Tg(Col13a1)2Pih |
Information from provider
| Provider | Taina Pihlajaniemi |
| Provider affiliation | Faculty of Biochemistry and Molecular medicine, University of Oulu |
| Genetic information | The construct is 4,7 kb in length. It consists of the following fragments. First fragment is a 2,5 kb starting from -984 (with respect to the ATG codon) and thus containing the Col13a1 promoter, and the first protein coding exon as well as 0,8 kb of following sequences in the first intron. This is linked to the second genomic fragment containing 159 bp in 3' of the first intron and 91 bp of the second exon. This is further linked to a Col13a1 cDNA clone containing rest of the second exon and exons 3-41. Finally, sequences coding for a hemagglutinin (HA) tag, transcription termination and SV40 polyA are linked 3' from the cDNA clone. |
| Phenotypic information | Homozygous:Transgenic female mice are not used for breeding due to the high bone mass phenotype and concomitant problems in delivery.Heterozygous:High bone mass apparent from 3-4 weeks onwards in transgenics. Most noticeable in proximal long bones but also calvarial bones are affected. |
| Breeding history | The construct was microinjected into one-cell stage embryos from B6D2F1 (hybrid of C57BL/6J x DBA/2J). The line was maintained as hybrid for about a decade, after which it was frozen using C57BL/6JOlaHsd females. The line was recovered and maintained thereafter using C57BL/6JOlaHsd females. |
| References |
|
| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Oulu, Oulu, Finland |
Literature references
- Type XIII collagen strongly affects bone formation in transgenic mice.;Ylönen Riikka, Kyrönlahti Tuomo, Sund Malin, Ilves Mika, Lehenkari Petri, Tuukkanen Juha, Pihlajaniemi Taina, ;2005;Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research;20;1381-93; 16007336
- Integrin α11β1 is a receptor for collagen XIII.;Koivunen Jarkko, Tu Hongmin, Kemppainen Antti, Anbazhagan Padmanabhan, Finnilä Mikko A, Saarakkala Simo, Käpylä Jarmo, Lu Ning, Heikkinen Anne, Juffer André H, Heino Jyrki, Gullberg Donald, Pihlajaniemi Taina, ;2021;Cell and tissue research;383;1135-1153; 33306155
- Collagen XIII-derived ectodomain regulates bone angiogenesis and intracortical remodeling.;Koivunen Jarkko, Kemppainen Antti V, Finnilä Mikko A, Keski-Filppula Riikka, Härönen Heli, Tu Hongmin, Pellikka Henri, Heikkinen Anne, Kylmäoja Elina, Sormunen Raija, Miinalainen Ilkka, Saarakkala Simo, Izzi Valerio, Pihlajaniemi Taina, ;2019;Matrix biology : journal of the International Society for Matrix Biology;83;6-25; 31220558