MGI phenotypes (allele matching)
FVB.129P2-Airntm1Dpb/Biat
| Status | Available to order |
| EMMA ID | EM:09895 |
| Citation information | RRID:IMSR_EM:09895 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | FVB.129P2-Airntm1Dpb/Biat |
| Alternative name | FVB.129P2-Airn tm1Dpb |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Airntm1Dpb |
| Gene/Transgene symbol | Airn |
Information from provider
| Provider | Denise Barlow |
| Provider affiliation | CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences |
| Genetic information | The AirnT allele contains a 1.2kb fragment from the rabbit beta-globin gene (bp 31392-32590; M18818) inserted 3kb downstream from the Airn TSS that causes the Airn long non-coding (lnc) RNA to be truncated and lose its imprinted silencing function. A 9.6kb BglII fragment from cosmid 940PS (129/Sv) containing the Airn promoter (mm10 chr17:12,740,025-12,749,644) was used to make the targeting construct. Targeting was done in E14 ES cells (129/Ola) and ES cells were injected into C57BL/6 blastocysts to generate chimeric mice. |
| Phenotypic information | Homozygous:The phenotype of homozygous mice is the same as for heteroyzgous mice with paternal inheritance, as Airn is only expressed from the paternal allele.Heterozygous:Heterozygotes with a paternally inherited AirnT allele show a 15% reduction in birth weight due to biallelic expression of Igf2r, as truncated Airn can no longer silence the paternal copy. Maternal heterozygotes show no phenotype, as Airn is not expressed from the maternal allele. |
| Breeding history | AirnT mice were derived from 129/Sv x C57BL/6 chimeras and then backcrossed to FVB/N for over 50 generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
| Animals used for archiving | heterozygous FVB.129P2/OlaHsd males |
Disease and phenotype information
Literature references
- The non-coding Air RNA is required for silencing autosomal imprinted genes.;Sleutels Frank, Zwart Ronald, Barlow Denise P, ;2002;Nature;415;810-3; 11845212
- Extra-embryonic-specific imprinted expression is restricted to defined lineages in the post-implantation embryo.;Hudson Quanah J, Seidl Christine I M, Kulinski Tomasz M, Huang Ru, Warczok Katarzyna E, Bittner Romana, Bartolomei Marisa S, Barlow Denise P, ;2011;Developmental biology;353;420-31; 21354127
- The Air noncoding RNA epigenetically silences transcription by targeting G9a to chromatin.;Nagano Takashi, Mitchell Jennifer A, Sanz Lionel A, Pauler Florian M, Ferguson-Smith Anne C, Feil Robert, Fraser Peter, ;2008;Science (New York, N.Y.);322;1717-20; 18988810
- Active and repressive chromatin are interspersed without spreading in an imprinted gene cluster in the mammalian genome.;Regha Kakkad, Sloane Mathew A, Huang Ru, Pauler Florian M, Warczok Katarzyna E, Melikant Balázs, Radolf Martin, Martens Joost H A, Schotta Gunnar, Jenuwein Thomas, Barlow Denise P, ;2007;Molecular cell;27;353-66; 17679087