B6.Cg-Tg(PECAM1-TFPI/CD4/SELP)#aAdor/AdorH

Status	Available to order
EMMA ID	EM:08452
Citation information	RRID:IMSR_EM:08452 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.Cg-Tg(PECAM1-TFPI/CD4/SELP)#aAdor/AdorH
Alternative name	CD31-TFPI-Tg (also known as XCON6)
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(PECAM1-TFPI/CD4/SELP)#aAdor
Gene/Transgene symbol	Tg(PECAM1-TFPI/CD4/SELP)#aAdor

Information from provider

Provider	Anthony Dorling
Provider affiliation	Innate Immunity, King's College London
Additional owner	Mark Thursz, Imperial College London, UK and Li Ke, Xi'an Jiatong University, China
Genetic information	Human tissue factor pathway inhibitor/CD4/P-selectin construct, with a modified CD31 promoter, introduced by microinjection. Copy number originally >30.
Phenotypic information	Homozygous: Mice express membrane tethered human TFPI-CD4 fusion protein on all CD31+ cells, which includes endothelial cells, monocytes and platelets. In all these, it is internalised within secretory granules at resting state by virtue of P-selectin cytoplasmic sequence. The fusion protein appears on the membrane on activation. The hTFPI inhibits tissue factor and FXa. Heterozygous: Exactly as homozygous.
Breeding history	Started backcrossing onto C57BL/6 in 2003, and then bred as sibling pairs since 2009.
References	Human tissue factor pathway inhibitor fused to CD4 binds both FXa and TF/FVIIa at the cell surface.;Riesbeck K, Dorling A, Kemball-Cook G, McVey J H, Jones M, Tuddenham E G, Lechler R I, ;1997;Thrombosis and haemostasis;78;1488-94; 9423800 Inhibition of tissue factor-dependent and -independent coagulation by cell surface expression of novel anticoagulant fusion proteins.;Chen D, Riesbeck K, Kemball-Cook G, McVey J H, Tuddenham E G, Lechler R I, Dorling A, ;1999;Transplantation;67;467-74; 10030296 Regulated inhibition of coagulation by porcine endothelial cells expressing P-selectin-tagged hirudin and tissue factor pathway inhibitor fusion proteins.;Chen D, Riesbeck K, McVey J H, Kemball-Cook G, Tuddenham E G, Lechler R I, Dorling A, ;1999;Transplantation;68;832-9; 10515384 Human thrombin and FXa mediate porcine endothelial cell activation; modulation by expression of TFPI-CD4 and hirudin-CD4 fusion proteins.;Chen D, Riesbeck K, McVey J H, Kemball-Cook G, Tuddenham E G, Lechler R I, Dorling A, ;2001;Xenotransplantation;8;258-65; 11737851 Inhibition of intravascular thrombosis in murine endotoxemia by targeted expression of hirudin and tissue factor pathway inhibitor analogs to activated endothelium.;Chen Daxin, Giannopoulos Konstantinos, Shiels Paul G, Webster Zoe, McVey John H, Kemball-Cook Geoff, Tuddenham Edward, Moore Marilyn, Lechler Robert, Dorling Anthony, ;2004;Blood;104;1344-9; 15126322 Complete inhibition of acute humoral rejection using regulated expression of membrane-tethered anticoagulants on xenograft endothelium.;Chen Daxin, Weber Michele, McVey John H, Kemball-Cook Geoffrey, Tuddenham Edward G D, Lechler Robert I, Dorling Anthony, ;2004;American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons;4;1958-63; 15575897 NK-cell-dependent acute xenograft rejection in the mouse heart-to-rat model.;Chen Daxin, Weber Michele, Lechler Robert, Dorling Anthony, ;2006;Xenotransplantation;13;408-14; 16925664 Protease-activated receptor 1 activation is necessary for monocyte chemoattractant protein 1-dependent leukocyte recruitment in vivo.;Chen Daxin, Carpenter Adam, Abrahams Joel, Chambers Rachel C, Lechler Robert I, McVey John H, Dorling Anthony, ;2008;The Journal of experimental medicine;205;1739-46; 18606855
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Literature references

Human tissue factor pathway inhibitor fused to CD4 binds both FXa and TF/FVIIa at the cell surface.;Riesbeck K, Dorling A, Kemball-Cook G, McVey J H, Jones M, Tuddenham E G, Lechler R I, ;1997;Thrombosis and haemostasis;78;1488-94; 9423800
Inhibition of tissue factor-dependent and -independent coagulation by cell surface expression of novel anticoagulant fusion proteins.;Chen D, Riesbeck K, Kemball-Cook G, McVey J H, Tuddenham E G, Lechler R I, Dorling A, ;1999;Transplantation;67;467-74; 10030296
Regulated inhibition of coagulation by porcine endothelial cells expressing P-selectin-tagged hirudin and tissue factor pathway inhibitor fusion proteins.;Chen D, Riesbeck K, McVey J H, Kemball-Cook G, Tuddenham E G, Lechler R I, Dorling A, ;1999;Transplantation;68;832-9; 10515384
Human thrombin and FXa mediate porcine endothelial cell activation; modulation by expression of TFPI-CD4 and hirudin-CD4 fusion proteins.;Chen D, Riesbeck K, McVey J H, Kemball-Cook G, Tuddenham E G, Lechler R I, Dorling A, ;2001;Xenotransplantation;8;258-65; 11737851
Inhibition of intravascular thrombosis in murine endotoxemia by targeted expression of hirudin and tissue factor pathway inhibitor analogs to activated endothelium.;Chen Daxin, Giannopoulos Konstantinos, Shiels Paul G, Webster Zoe, McVey John H, Kemball-Cook Geoff, Tuddenham Edward, Moore Marilyn, Lechler Robert, Dorling Anthony, ;2004;Blood;104;1344-9; 15126322
Complete inhibition of acute humoral rejection using regulated expression of membrane-tethered anticoagulants on xenograft endothelium.;Chen Daxin, Weber Michele, McVey John H, Kemball-Cook Geoffrey, Tuddenham Edward G D, Lechler Robert I, Dorling Anthony, ;2004;American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons;4;1958-63; 15575897
NK-cell-dependent acute xenograft rejection in the mouse heart-to-rat model.;Chen Daxin, Weber Michele, Lechler Robert, Dorling Anthony, ;2006;Xenotransplantation;13;408-14; 16925664
Protease-activated receptor 1 activation is necessary for monocyte chemoattractant protein 1-dependent leukocyte recruitment in vivo.;Chen Daxin, Carpenter Adam, Abrahams Joel, Chambers Rachel C, Lechler Robert I, McVey John H, Dorling Anthony, ;2008;The Journal of experimental medicine;205;1739-46; 18606855

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