IMPC phenotypes (gene matching)
129-Grin1tm1.1Grnt/WtsiH
| Status | Available to order |
| EMMA ID | EM:15856 |
| Citation information | RRID:IMSR_EM:15856 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | 129-Grin1tm1.1Grnt/WtsiH |
| Alternative name | 129-Grin1 |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Grin1tm1.1Grnt |
| Gene/Transgene symbol | Grin1 |
Information from provider
| Provider | Seth Grant |
| Provider affiliation | Wellcome Trust Sanger Institute |
| Genetic information | Information sourced from https://doi.org/10.1038/ncomms11264. This Grin1 knock in strain was created by inserting a tandem affinity purification (TAP) tag into the first protein-coding exon of the GluN1 subunit gene (Glun1) by homologous recombination in embryonic stem cells. The TAP tag consists of a linker, a 3xFlag epitope tag, linker containing a TEV protease consensus site, hexa-histidine tag and a linker to the beginning of the amino-terminal domain. |
| Phenotypic information | On biochemical, anatomical, and electrophysiological assays, the receptor functioned normally. |
| Breeding history | Established from crossing hets from GER (AE1) with CAG-Cre (GBT) mice to remove neo cassette. Maintained as Het x GPAA (129), Het x Het and then Hom x Hom intercrosses. Hom viable at 37% and Hom fertile. Maintained on a mixed background- 129S5/SvEvBrdWtsi 129P2/OlaHsdWtsi |
| References |
|
| Homozygous fertile | not known |
| Homozygous viable | yes |
| Homozygous matings required | not known |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Autosomal dominant non-syndromic intellectual disability / Orphanet_178469
- Bilateral generalized polymicrogyria / Orphanet_208447
- Early infantile epileptic encephalopathy / Orphanet_1934
MGI phenotypes (gene matching)
- abnormal trigeminal nerve morphology / MGI
- atelectasis / MGI
- lung hemorrhage / MGI
- overexpanded pulmonary alveoli / MGI
- decreased body weight / MGI
- abnormal social investigation / MGI
- social withdrawal / MGI
- abnormal anxiety-related response / MGI
- reduced male mating frequency / MGI
- abnormal pup retrieval / MGI
- pup cannibalization / MGI
- abnormal maternal nurturing / MGI
- abnormal locomotor behavior / MGI
- ataxia / MGI
- hyperactivity / MGI
- hypoactivity / MGI
- impaired coordination / MGI
- stereotypic behavior / MGI
- increased exploration in new environment / MGI
- absent suckling reflex / MGI
- abnormal suckling behavior / MGI
- aphagia / MGI
- abnormal nest building behavior / MGI
- abnormal huddling behavior / MGI
- abnormal spatial learning / MGI
- reduced long term potentiation / MGI
- increased startle reflex / MGI
- impaired righting response / MGI
- cyanosis / MGI
- postnatal growth retardation / MGI
- absence of NMDA-mediated synaptic currents / MGI
- reduced male fertility / MGI
- abnormal respiration / MGI
- abnormal breathing pattern / MGI
- respiratory distress / MGI
- apnea / MGI
- abnormal motor capabilities/coordination/movement / MGI
- premature death / MGI
- no abnormal phenotype detected / MGI
- abnormal nervous system electrophysiology / MGI
- abnormal social/conspecific interaction / MGI
- decreased vertical activity / MGI
- abnormal conditioned taste aversion behavior / MGI
- abnormal glutamate-mediated receptor currents / MGI
- decreased susceptibility to pharmacologically induced seizures / MGI
- abnormal NMDA-mediated synaptic currents / MGI
- no phenotypic analysis / MGI
- thrombocytopenia / MGI
- increased alcohol consumption / MGI
- decreased alcohol consumption / MGI
- nervous system phenotype / MGI
- abnormal nervous system physiology / MGI
- decreased aggression towards mice / MGI
- impaired passive avoidance behavior / MGI
- abnormal miniature excitatory postsynaptic currents / MGI
- abnormal neuron physiology / MGI
- abnormal brainstem morphology / MGI
- cardiovascular system phenotype / MGI
- behavior/neurological phenotype / MGI
- taste/olfaction phenotype / MGI
- abnormal food intake / MGI
- abnormal pulmonary respiratory rate / MGI
- decreased susceptibility to neuronal excitotoxicity / MGI
- abnormal physiological response to xenobiotic / MGI
- decreased prepulse inhibition / MGI
- abnormal maternal grooming / MGI
- absent gastric milk in neonates / MGI
- abnormal behavioral response to xenobiotic / MGI
- reduced AMPA-mediated synaptic currents / MGI
- postnatal lethality, complete penetrance / MGI
- postnatal lethality, incomplete penetrance / MGI
- neonatal lethality, complete penetrance / MGI
- perinatal lethality, complete penetrance / MGI
- perinatal lethality, incomplete penetrance / MGI
- abnormal behavioral response to alcohol / MGI
Literature references
- NMDA receptors are selectively partitioned into complexes and supercomplexes during synapse maturation.;Frank René A W, Komiyama Noboru H, Ryan Tomás J, Zhu Fei, O'Dell Thomas J, Grant Seth G N, ;2016;Nature communications;7;11264; 27117477