B6;129-Lta/Tnftm1Fda/Orl

Status

Available to order

EMMA IDEM:00130
International strain nameB6;129-Lta/Tnftm1Fda/Orl
Alternative nameLT-a TNF-a
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolLta/Tnftm1Fda,
Gene/Transgene symbolLta

Information from provider

ProviderF Dautry
Provider affiliationc/o CDTA-Orleans
Phenotypic informationMutant mice deficient in lymphotoxin A (Lta or LT-alpha) and tumor necrosis factors (Tnf or TNF-alpha) genes, by homologous recombination (insertion of neo gene with deletion of all the Lta gene and a part of the Tnf gene). The tumor necrosis factors (TNF and LTA) are important mediators of the immune and inflammatory responses. The heterozygous mice have 20- to 100-fold lower TNF levels in circulation than the wild-type mice. Moreover, the heterozygous animals have an increased bacterial load following Listeria monocytogenes infection.
Breeding historyR1 ES cells (129/Sv); chimaeras crossed to C57BL/6 and then intercrossed.
References
  • Mice heterozygous for a deletion of the tumor necrosis factor-alpha and lymphotoxin-alpha genes: biological importance of a nonlinear response of tumor necrosis factor-alpha to gene dosage.;Amiot F, Boussadia O, Cases S, Fitting C, Lebastard M, Cavaillon J M, Milon G, Dautry F, ;1997;European journal of immunology;27;1035-42; 9130661

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France

Disease and phenotype information

MGI phenotypes (allele matching)
  • increased susceptibility to bacterial infection / MGI
  • decreased tumor necrosis factor secretion / MGI
  • decreased susceptibility to endotoxin shock / MGI

Literature references

  • Mice heterozygous for a deletion of the tumor necrosis factor-alpha and lymphotoxin-alpha genes: biological importance of a nonlinear response of tumor necrosis factor-alpha to gene dosage.;Amiot F, Boussadia O, Cases S, Fitting C, Lebastard M, Cavaillon J M, Milon G, Dautry F, ;1997;European journal of immunology;27;1035-42; 9130661

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.


Search
INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).