B6;129-Col15a1tm2.1Pih Tg(Tie1-cre)9Ref/Oulu

Status

Available to order

EMMA IDEM:10618
International strain nameB6;129-Col15a1tm2.1Pih Tg(Tie1-cre)9Ref/Oulu
Alternative nameEndothelial cell-specific collagen XV knockout in B6 background
Strain typeTargeted Mutant Strains : Conditional mutation
Allele/Transgene symbolCol15a1tm2.1Pih, Tg(Tie1-cre)9Ref
Gene/Transgene symbolCol15a1, Tg(Tie1-cre)9Ref

Information from provider

ProviderTaina Pihlajaniemi
Provider affiliationFaculty of Biochemistry and Molecular Medicine, University of Oulu
Additional ownerTg(Tie1-cre)9Ref originates from Prof. Reinhard Fässler, Max Planck Institute of Biochemistry, Martinsried, Germany.
Genetic informationCol15a1 contains two loxP sites, one 900 bp upstream of the first exon and a second one 120 bp downstream of the second exon. Cre-mediated recombination of neo+tk selection cassette out was performed in ES cells. Col15a1 floxed mice were bred with the endothelial cell-specific cre recombinase-expressing line, Tg(Tie1-cre)9Ref.
Phenotypic informationHomozygous:
No obvious phenotype

Heterozygous:
Not observed
Breeding historyMice were bred 2-3 generations with C57BL/6J mice, cross-bred with Tg(Tie1-cre)9Ref in C57BL/6J background.
References
  • Lack of type XV collagen causes a skeletal myopathy and cardiovascular defects in mice.;Eklund L, Piuhola J, Komulainen J, Sormunen R, Ongvarrasopone C, Fássler R, Muona A, Ilves M, Ruskoaho H, Takala T E, Pihlajaniemi T, ;2001;Proceedings of the National Academy of Sciences of the United States of America;98;1194-9; 11158616
  • Lack of type XV collagen causes a skeletal myopathy and cardiovascular defects in mice.;Eklund L, Piuhola J, Komulainen J, Sormunen R, Ongvarrasopone C, Fássler R, Muona A, Ilves M, Ruskoaho H, Takala T E, Pihlajaniemi T, ;2001;Proceedings of the National Academy of Sciences of the United States of America;98;1194-9; 11158616
  • Tie-1-directed expression of Cre recombinase in endothelial cells of embryoid bodies and transgenic mice.;Gustafsson E, Brakebusch C, Hietanen K, Fässler R, ;2001;Journal of cell science;114;671-6; 11171372
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreUniversity of Oulu, Oulu, Finland
Animals used for archivingheterozygous C57BL/6JOlaHsd, wild-type C57BL/6JOlaHsd
Stage of embryosMorula

Disease and phenotype information

MGI phenotypes (gene matching)
  • abnormal muscle regeneration / MGI
  • myopathy / MGI
  • abnormal cardiovascular system physiology / MGI
  • abnormal muscle morphology / MGI
  • abnormal capillary morphology / MGI
  • abnormal muscle fiber morphology / MGI
  • abnormal vascular endothelial cell morphology / MGI

Literature references

  • Lack of type XV collagen causes a skeletal myopathy and cardiovascular defects in mice.;Eklund L, Piuhola J, Komulainen J, Sormunen R, Ongvarrasopone C, Fássler R, Muona A, Ilves M, Ruskoaho H, Takala T E, Pihlajaniemi T, ;2001;Proceedings of the National Academy of Sciences of the United States of America;98;1194-9; 11158616
  • Tie-1-directed expression of Cre recombinase in endothelial cells of embryoid bodies and transgenic mice.;Gustafsson E, Brakebusch C, Hietanen K, Fässler R, ;2001;Journal of cell science;114;671-6; 11171372

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

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Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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