Available to order

International strain name129S4(B6N)-Slc1a3tm1.1(cre/ERT2)Wsj/WsjH
Alternative nameSlc1a3-CreERT2
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolSlc1a3tm1.1(cre/ERT2)Wsj,
Gene/Transgene symbolSlc1a3

Information from provider

ProviderWalker Jackson
Provider affiliationClinical and Experimental Medicine, Linköping University
Genetic informationSequence encoding the Cre-ERT2 fusion protein was inserted in place of the stop codon of the sequence encoding Slc1a3. A 2A peptide separates the two proteins during translation.
Phenotypic informationHomozygous:
This line expresses Cre-ERT2 from the Slc1a3 (or GLAST) locus, leaving the native protein coding sequence intact. The mice appear normal as homozygotes. The oldest age observed is 6 months. The line is still new so not fully characterized. Homozygotes are easily distinguished from heterozygotes by the standard PCR assay.

Heterozygotes appear no different from homozygotes or wild-type mice
Breeding historyThe allele was targeted into J1 129S4 ES cells. Chimeras were bred to mice expressing Flp recombinase (from the ROSA26 locus) to remove the selectable marker (neo) that was inserted approximately 200 base pairs downstream of the stop codon. The Flp mice were obtained from Jax on a 129S4 background and were bred in house to carry wild-type Disc1 and the non-agouti gene from C57BL/6N mice. The final genetic background of the Flp mice is >99% 129S4, verified by SNP analysis. The F1 and F2 generations were bred to 129S4 to reintroduce natural alleles of Disc1, agouti, and ROSA26 from 129S4. F3 and F4 generations were intercrossed to generate and expand a homozygous line. They currently exist at the F5 generation.
ReferencesNone available
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

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Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

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