STOCK Hrhr Alox12btm1Krie Krt14tm1.1(cre/ERT2)Hjg/KrieH


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International strain nameSTOCK Hrhr Alox12btm1Krie Krt14tm1.1(cre/ERT2)Hjg/KrieH
Alternative nameSKH1/Alox12bflox/K14CreJ
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolKrt14tm1.1(cre/ERT2)Hjg,
Gene/Transgene symbolKrt14

Information from provider

ProviderPeter Krieg
Provider affiliationDeutsches Krebsforschungszentrum
Additional ownerProf. Pierre Chambon, IGBMC, Illkirch, France
Genetic information1. Generation of mice harboring loxP containing Alox12b alleles (Epp et al., 2003): by using a 7.4-kb BamHI fragment from a 129/Ola mouse genomic PAC library and a 4.4-kb PCR-generated fragment containing exon 7–12, a homology region was cloned into pBluescript KS+ that spanned from 226 nt upstream of exon 3 to 1081 nt downstream of exon 11. A tk/neo selection cassette flanked by two loxP sites was inserted 230 bp upstream of exon 8, replacing a 222-bp AccI fragment; a third loxP site was inserted 84 bp downstream of exon 8, deleting a BamHI site. The linearized targeting vector was electroporated into ES cells. ES cells were injected into C57BL/6 blastocysts to generate chimeric mice, which were mated with C57BL/6 females. F1 heterozygotes (Alox12b+/flox) were then crossed to 129S6. 2. Generation of conditional Alox12b knockout mice: mice harboring loxP containing Alox12b alleles were crossed with CMV-cre deleter mice to remove Tk/neo resistance cassette and to obtain Alox12b+/fl mice in which exon 8 of Alox12b is flanked by two loxP sites. Alox12bfl/fl mice were crossed to mice expressing cre recombinase fused to the human estrogen receptor under the control of a truncated keratin 14 promoter (Amen et al., 2013) to obtain mice with Tamoxifen-inducible, epidermis-specific deletion of Alox12b (Alox12bfl/fl K14CreJ mice). The Alox12bfl/fl K14CreJ mice were backcrossed to the hairless SKH1 background for at least five generations.
Phenotypic informationHomozygous:
Complete ablation of Alox12b in epidermis and other keratin 14-expressing tissues.

Ablation of one copy of Alox12b allele in epidermis and other keratin 14-expressing tissues.
Breeding historyThis line was originally 129S6 but later it was back-crossed for about five generations to SKH1 (Hrhr).
  • The role of lipoxygenases in epidermis.;Krieg Peter, Fürstenberger Gerhard, ;2014;Biochimica et biophysica acta;1841;390-400; 23954555
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Literature references

  • The role of lipoxygenases in epidermis.;Krieg Peter, Fürstenberger Gerhard, ;2014;Biochimica et biophysica acta;1841;390-400; 23954555

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