B6.Cg-Il6tm2.1Jho/Cnbc
| Status | Available to order |
| EMMA ID | EM:13231 |
| International strain name | B6.Cg-Il6tm2.1Jho/Cnbc |
| Alternative name | IL6-DIO-KO (Il6 |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Il6tm2.1Jho, |
| Gene/Transgene symbol | Il6 |
Information from provider
| Provider | Juan Hidalgo |
| Provider affiliation | Biologia Celular, Fisiologia e Inmunologia, Universidad Autonoma de Barcelona |
| Genetic information | The gene is interleukin 6 (Il6). This is a conditional reversible Il6 knock-out (IL6-DIO-KO) mouse line that was developed using double-inverted open reading frame (DIO) technology. |
| Phenotypic information | Homozygous:The animal will be systemic Il6 knock-out (which can be reactivated by cre recombinase). In normal conditions will breed normally and there is no obvious phenotype. A clear phenotype will be appearing in specific experimental settings. For instance, the mice will be resistant to the experimental autoimmune encephalomyelitis (EAE) multiple sclerosis model.Heterozygous:The animal will have one wild-type Il6 allele and a null allele. |
| Breeding history | The mutant mice have been backcrossed >10 times to C57BL/6OlaHsd mice. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | yes |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous C57BL/6JOlaHsd |
Literature references
- A new mouse model to study restoration of interleukin-6 (IL-6) expression in a Cre-dependent manner: microglial IL-6 regulation of experimental autoimmune encephalomyelitis.;Sanchis Paula, Fernández-Gayol Olaya, Comes Gemma, Aguilar Kevin, Escrig Anna, Giralt Mercedes, Palmiter Richard D, Hidalgo Juan, ;2020;Journal of neuroinflammation;17;304; 33059703