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International strain nameB6J;B6N-Nkx1-2tm2296.1(cre/ERT2)Arte/H
Alternative nameNkx1-2CreERT2
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolNkx1-2tm2296.1(cre/ERT2)Arte,
Gene/Transgene symbolNkx1-2

Information from provider

ProviderJames Briscoe
Provider affiliationThe Francis Crick Institute
Genetic informationIn this mouse line creERT2 recombinase expression is driven under the control of the endogenous promoter of the transcription factor Nkx1-2. The targeting strategy was designed to replace Nkx1-2 exon 1 and the splice donor site at the junction between exon 1 and intron 1 with a cassette containing the open reading frame of creERT2 (see Rodrigo-Albors et al. Development, 2018, 145, dev164319; doi:10.1242/dev.164319).
Phenotypic informationHomozygous:
creERT2 will be expressed from the endogenous Nkx1-2 promoter and should thus recapitulate the expression pattern of the Nkx1-2 gene. The replacement of the Nkx1-2 coding sequence in exon 1 with the creERT2 and the polyadenylation signal should result in the loss of function of the Nkx1-2 gene. However, loss of function of the Nkx1-2 (aka Sax1) gene did not generate a phenotype in either heterozygous or homozygous mice. This is likely due to genetic compensation or functional redundancy by another related gene/s and confirms previous findings, see Schubert, F. R., Fainsod, A., Gruenbaum, Y. and Gruss, P., 1995, Expression of the novel murine homeobox gene Sax-1 in the developing nervous system, Mech. Dev. 51, 99-114.

Consistent with the Schubert et al. 1995 study; no phenotype has been observed with this reporter line.
Breeding historyTaconic provided two breeding pairs of heterozygous C57BL/6–Nkx1-2tm2296(Cre-ER(T2))Arte mice. These mice carried a puromycin-expressing cassette flanked by F3 sites, which was removed upon crossing to flp recombinase-expressing mice. The resulting mice express creERT2 from the endogenous Nkx1-2 promoter (Nkx1-2creERT2). Nkx1-2CreERT2 mice were then bred to homozygosity to establish a breeding colony. We maintained this homozygous Nkx1-2CreERT2 colony for more than nine generations without any obvious deleterious effects. Genetic background: C57BL/6NTac and C57BL/6J mixed.
  • Lineage tracing of axial progenitors using Nkx1-2CreERT2 mice defines their trunk and tail contributions.;Rodrigo Albors Aida, Halley Pamela A, Storey Kate G, ;2018;Development (Cambridge, England);145;; 30201686
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisednot known

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Literature references

  • Lineage tracing of axial progenitors using Nkx1-2CreERT2 mice defines their trunk and tail contributions.;Rodrigo Albors Aida, Halley Pamela A, Storey Kate G, ;2018;Development (Cambridge, England);145;; 30201686

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