Available to order

International strain nameC57BL/6N-Mcpt8tm2.2(RFP657/cre)Ics/Ics
Alternative nameMcpt8tm2.2(RFP657/cre)Ics
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolMcpt8tm2.2(RFP657/cre)Ics
Gene/Transgene symbolMcpt8

Information from provider

Provider ICS, Institut Clinique de la Souris
Provider affiliationICS, Institut Clinique de la Souris
Genetic informationThis line was obtained by crossing Mcpt8tm2.1(RFP657/cre/ERT2)Ics with the C57BL/6NTac-Gt(ROSA)26Sortm2(CAG-flpo,-EYFP)Ics Flp ubiquitous deleter (EMMA strain ID EM:05490), resulting in an RFP657-LF2A-cre-F3-Rox sequence located in exon 1 of Mcpt8. The pure C57BL/6N background was maintained for all breeding steps. FACS analysis showed efficient and specific cre expression in spleen and skin basophils after breeding with the Gt(ROSA)26Sortm1Hjf reporter line. TagRFP657 is not detected due to the mutation in its cDNA. The detailed model generation technical report is enclosed. For detailed information on the genetic description of this strain, please have a look at this report.
Phenotypic informationHomozygous:

Breeding historyC57BL/6N inbred (4 generations).
ReferencesNone available
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requiredno

Information from EMMA

Archiving centreICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France

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Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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