C57BL/6N-Twist1tm1.1Arte/Ieg

Status

Available to order

EMMA IDEM:05504
Citation informationRRID:IMSR_EM:05504 

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International strain nameC57BL/6N-Twist1tm1.1Arte/Ieg
Alternative nameTwist1 KO cond
Strain typeTargeted Mutant Strains : Conditional mutation
Allele/Transgene symbolTwist1tm1.1
Gene/Transgene symbolTwist1

Information from provider

Provider Helmholtz Zentrum Muenchen
Provider affiliationInstitute of Experimental Genetics, Helmholtz Zentrum Muenchen German Research Center for Environmental Health (GmbH)
Genetic informationThe complete mouse Twist1 ORF is encoded by exon 1. Exon 1 including 1.5 kb of the promoter region has been flanked by loxP sites, as its genetic ablation should result in loss of function. The conditional targeted mutation has been obtained by in vivo, Flp recombinase-mediated removal of the neomycin selection marker.
Phenotypic informationTO BE PROVIDED
Breeding historyTargeting performed in C57BL/6N ES cells and confirmed by Southern blotting. Crossed to C57BL/6N-Tg(CAG-flpe)2Arte mice to obtain mutant founders with excised neo cassette. Maintained on C57BL/6N background.
ReferencesNone available
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requirednot known
Immunocompromisednot known

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivingheterozygous C57BL/6NTac males, wild-type C57BL/6N females
Stage of embryos2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)
  • abnormal digit morphology / IMPC
  • abnormal autopod morphology / IMPC
  • abnormal zygomatic bone morphology / IMPC
  • abnormal pelvic girdle bone morphology / IMPC
  • abnormal brain morphology / IMPC
  • abnormal nail morphology / IMPC
  • increased lean body mass / IMPC
  • abnormal vertebrae morphology / IMPC
  • abnormal vertebral arch morphology / IMPC
  • increased grip strength / IMPC
  • decreased prepulse inhibition / IMPC
  • decreased total body fat amount / IMPC
  • abnormal cranium morphology / IMPC
MGI phenotypes (gene matching)
  • premature cranial suture closure / MGI
  • cleft palate / MGI
  • abnormal rib morphology / MGI
  • short limbs / MGI
  • abnormal forelimb morphology / MGI
  • polydactyly / MGI
  • abnormal muscle development / MGI
  • exencephaly / MGI
  • open neural tube / MGI
  • decreased body size / MGI
  • abnormal gait / MGI
  • internal hemorrhage / MGI
  • abnormal limb morphology / MGI
  • abnormal limb bone morphology / MGI
  • premature endochondral bone ossification / MGI
  • nervous system phenotype / MGI
  • enlarged interparietal bone / MGI
  • temporal bone hypoplasia / MGI
  • abnormal clavicle morphology / MGI
  • craniofacial phenotype / MGI
  • skeleton phenotype / MGI
  • abnormal bone ossification / MGI
  • abnormal osteoblast differentiation / MGI
  • short temporal bone squamous part / MGI
  • neonatal lethality, complete penetrance / MGI
  • embryonic lethality, complete penetrance / MGI
  • prenatal lethality, incomplete penetrance / MGI
  • premature coronal suture closure / MGI
  • premature lambdoid suture closure / MGI

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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