STOCK Cdk2^tm2Sgo/Cnbc

Status	Available to order
EMMA ID	EM:05931
Citation information	RRID:IMSR_EM:05931 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	STOCK Cdk2^tm2Sgo/Cnbc
Alternative name	Cdk2
Strain type	Targeted Mutant Strains : Conditional mutation
Allele/Transgene symbol	Cdk2^tm2Sgo
Gene/Transgene symbol	Cdk2

Information from provider

Provider	Mariano Barbacid
Provider affiliation	Molecular Oncology, Centro Nacional de Investigaciones Oncologicas
Genetic information	A neomycin resistance gene flanked by loxP sites was originally inserted into intron 3 while a third loxP site was inserted into intron 1. The neomycin resistance gene was then excised by Cre mediated recombination so that exons 2 and 3 are flanked by loxP sites.
Phenotypic information	Before Cre mediated recombination there is no phenotype associated to this allele.
Breeding history	Chimeras were crossed with CD1 females for germ line transmission analysis. Heterozygous offspring was crossed with Tg.EIIaCre (FVB/N-TgN(EIIa-Cre)C5379Lmgd) for neomycin resistance gene excision. Offspring was intercrossed to obtain homozygous animals for the Cdk2_lox allele and maintained in homozygosis.
References	Cyclin-dependent kinase 2 is essential for meiosis but not for mitotic cell division in mice.;Ortega Sagrario, Prieto Ignacio, Odajima Junko, Martín Alberto, Dubus Pierre, Sotillo Rocio, Barbero Jose Luis, Malumbres Marcos, Barbacid Mariano, ;2003;Nature genetics;35;25-31; 12923533 Mice thrive without Cdk4 and Cdk2.;Barrière Cédric, Santamaría David, Cerqueira Antonio, Galán Javier, Martín Alberto, Ortega Sagrario, Malumbres Marcos, Dubus Pierre, Barbacid Mariano, ;2007;Molecular oncology;1;72-83; 19383288 Cdk1 is sufficient to drive the mammalian cell cycle.;Santamaría David, Barrière Cédric, Cerqueira Antonio, Hunt Sarah, Tardy Claudine, Newton Kathryn, Cáceres Javier F, Dubus Pierre, Malumbres Marcos, Barbacid Mariano, ;2007;Nature;448;811-5; 17700700 A synthetic lethal interaction between K-Ras oncogenes and Cdk4 unveils a therapeutic strategy for non-small cell lung carcinoma.;Puyol Marta, Martín Alberto, Dubus Pierre, Mulero Francisca, Pizcueta Pilar, Khan Gulfaraz, Guerra Carmen, Santamaría David, Barbacid Mariano, ;2010;Cancer cell;18;63-73; 20609353
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	homozygous mixed males

Disease and phenotype information

MGI phenotypes (gene matching)

abnormal female reproductive system morphology / MGI
abnormal oocyte morphology / MGI
abnormal ovary morphology / MGI
absent corpus luteum / MGI
abnormal male reproductive system morphology / MGI
small testis / MGI
small seminiferous tubules / MGI
abnormal spermatogenesis / MGI
decreased body weight / MGI
decreased body size / MGI
reduced male fertility / MGI
infertility / MGI
male infertility / MGI
female infertility / MGI
abnormal gametogenesis / MGI
abnormal meiosis / MGI
abnormal oogenesis / MGI
abnormal spermiogenesis / MGI
abnormal fertility/fecundity / MGI
abnormal seminiferous tubule morphology / MGI
absent ovarian follicles / MGI
abnormal cell cycle / MGI
testicular atrophy / MGI
decreased hepatocyte proliferation / MGI
absent oocytes / MGI
ovary atrophy / MGI
uterus atrophy / MGI
azoospermia / MGI
abnormal male meiosis / MGI
cellular phenotype / MGI
reproductive system phenotype / MGI
prenatal lethality, incomplete penetrance / MGI
preweaning lethality, incomplete penetrance / MGI

Literature references

Cyclin-dependent kinase 2 is essential for meiosis but not for mitotic cell division in mice.;Ortega Sagrario, Prieto Ignacio, Odajima Junko, Martín Alberto, Dubus Pierre, Sotillo Rocio, Barbero Jose Luis, Malumbres Marcos, Barbacid Mariano, ;2003;Nature genetics;35;25-31; 12923533
Mice thrive without Cdk4 and Cdk2.;Barrière Cédric, Santamaría David, Cerqueira Antonio, Galán Javier, Martín Alberto, Ortega Sagrario, Malumbres Marcos, Dubus Pierre, Barbacid Mariano, ;2007;Molecular oncology;1;72-83; 19383288
Cdk1 is sufficient to drive the mammalian cell cycle.;Santamaría David, Barrière Cédric, Cerqueira Antonio, Hunt Sarah, Tardy Claudine, Newton Kathryn, Cáceres Javier F, Dubus Pierre, Malumbres Marcos, Barbacid Mariano, ;2007;Nature;448;811-5; 17700700
A synthetic lethal interaction between K-Ras oncogenes and Cdk4 unveils a therapeutic strategy for non-small cell lung carcinoma.;Puyol Marta, Martín Alberto, Dubus Pierre, Mulero Francisca, Pizcueta Pilar, Khan Gulfaraz, Guerra Carmen, Santamaría David, Barbacid Mariano, ;2010;Cancer cell;18;63-73; 20609353
CDK2 inhibitors as candidate therapeutics for cisplatin- and noise-induced hearing loss.;Teitz Tal, Fang Jie, Goktug Asli N, Bonga Justine D, Diao Shiyong, Hazlitt Robert A, Iconaru Luigi, Morfouace Marie, Currier Duane, Zhou Yinmei, Umans Robyn A, Taylor Michael R, Cheng Cheng, Min Jaeki, Freeman Burgess, Peng Junmin, Roussel Martine F, Kriwacki Richard, Guy R Kiplin, Chen Taosheng, Zuo Jian, ;2018;The Journal of experimental medicine;215;1187-1203; 29514916

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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STOCK Cdk2tm2Sgo/Cnbc