IMPC phenotypes (gene matching)
STOCK Fzr1tm1Mama/Cnbc
| Status | Available to order |
| EMMA ID | EM:06108 |
| Citation information | RRID:IMSR_EM:06108 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | STOCK Fzr1tm1Mama/Cnbc |
| Alternative name | Frz1 |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Fzr1tm1Mama |
| Gene/Transgene symbol | Fzr1 |
Information from provider
| Provider | Marcos Malumbres |
| Provider affiliation | Molecular Oncology, Centro Nacional de Investigaciones Oncologicas (CNIO) |
| Genetic information | A loxP site was inserted upstream of exon 2 and an frt-flanked neo cassette with a 5' loxP site was inserted downstream of exon 3. Germ line, flp-mediated recombination was used to remove the neo cassette leaving exons 2 and 3 floxed; cre-mediated recombination between the two loxP sites eliminates exons 2 and 3. Splicing from exon 1 to exon 4 sequences causes a frameshift in the open reading frame that results in the synthesis of a putative small polypeptide unrelated to Fzr1. |
| Phenotypic information | Before cre-mediated recombination there is no phenotype associated to this allele. |
| Breeding history | Chimeras were crossed with C57BL/6J females for germ line transmission testing. To remove the neoR selection marker Fzr1+/loxfrt mice were crossed with Tg(ACTFLPe)9205Dym mice to remove the frt-flanked neomycin resistance cassette used for homologous recombination selection. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous mixed males |
Disease and phenotype information
MGI phenotypes (gene matching)
- decreased cell proliferation / MGI
- abnormal object recognition memory / MGI
- abnormal contextual conditioning behavior / MGI
- reduced long term potentiation / MGI
- abnormal motor coordination/balance / MGI
- decreased embryo size / MGI
- abnormal placenta morphology / MGI
- absent trophoblast giant cells / MGI
- placental labyrinth hypoplasia / MGI
- abnormal placenta labyrinth morphology / MGI
- increased mammary adenocarcinoma incidence / MGI
- increased tumor incidence / MGI
- premature death / MGI
- no phenotypic analysis / MGI
- decreased tumor growth/size / MGI
- binucleate / MGI
- embryonic growth retardation / MGI
- abnormal mitosis / MGI
- small placenta / MGI
- pale placenta / MGI
- abnormal postnatal subventricular zone morphology / MGI
- decreased incidence of tumors by chemical induction / MGI
- abnormal trophoblast giant cell morphology / MGI
- behavior/neurological phenotype / MGI
- increased testis tumor incidence / MGI
- increased lung tumor incidence / MGI
- increased liver tumor incidence / MGI
- embryonic lethality / MGI
- abnormal placental labyrinth vasculature morphology / MGI
- decreased erythroid progenitor cell number / MGI
- increased mammary gland tumor incidence / MGI
- mortality/aging / MGI
- embryonic lethality during organogenesis, complete penetrance / MGI
- decreased fibroblast cell migration / MGI
Literature references
- Genomic stability and tumour suppression by the APC/C cofactor Cdh1.;García-Higuera Irene, Manchado Eusebio, Dubus Pierre, Cañamero Marta, Méndez Juan, Moreno Sergio, Malumbres Marcos, ;2008;Nature cell biology;10;802-11; 18552834
- The APC/C activator FZR1 coordinates the timing of meiotic resumption during prophase I arrest in mammalian oocytes.;Holt Janet E, Tran Suzanne M-T, Stewart Jessica L, Minahan Kyra, García-Higuera Irene, Moreno Sergio, Jones Keith T, ;2011;Development (Cambridge, England);138;905-13; 21270054