B6N.Cg-Gt(ROSA)26Sorem1.1(CAG-Rpl22,-Uprt,-mKate2,-Ago2)Jaws/Orl
| Status | Only small colony available |
| EMMA ID | EM:14660 |
| International strain name | B6N.Cg-Gt(ROSA)26Sorem1.1(CAG-Rpl22,-Uprt,-mKate2,-Ago2)Jaws/Orl |
| Alternative name | B6.129S4-Gt(ROSA)26Sortm1.1Jaws |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Gt(ROSA)26Sorem1.1(CAG-Rpl22, -Uprt |
| Gene/Transgene symbol | Gt(ROSA)26Sor |
Information from provider
| Provider | Walker Jackson |
| Provider affiliation | Neurobiology, Linköping University |
| Additional owner | German Center for Neurodegenerative Diseases (DZNE) |
| Genetic information | Commonly called B6-LSL-Tagger. This is a knock-in mouse line bearing a quad-cistronic transgene, combining enrichment tools for nuclei, nascent RNA, translating mRNA, and mature microRNA (miRNA). Tagger captures whole nuclei for chromatin and nuclear RNA (Nuc-Tag), nascent RNA pulse labeled with 4-Thiouracil (TU-Tag), translating mRNA embedded in ribosomes (Ribo-Tag), and mature miRNA bound to the argonaute 2 protein (Ago-Tag). Following activation with cell type–specific cre- and/or Flp-driver lines, Tagger provides a platform to perform multiple omics experiments on specific cell populations in vivo, using the same mouse. The Tagger's transgene contains: ubiquitous synthetic CAG promoter; Frt-NeoR-Frt (FNF) and LoxP-STOP-LoxP (LSL) transcriptional STOP cassettes, flanked by sites for specific recombinases (Flp and cre, respectively), allowing cell type–specific, intersectional activation of expression; single ORF encoding four proteins (in colors) separated by 2A peptides: hemagglutinin-tagged large subunit ribosome protein 22 (Rpl22-HA, Ribo-Tag), Toxoplasma gondii uracil phosphoribosyltransferase (TgUPRT, TU-Tag), red fluorescent protein with three NLSs (RFP-NLS, Nuc-Tag), and FLAG- and V5-tagged argonaute 2 protein (FLAG-V5-Ago2, Ago-Tag); Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE); poly(A). CRISPR technology details: CRISPR/Cas9 nickase plasmid pX335 (a gift from Feng Zhang, plasmid #42335, Addgene, Cambridge, Massachusetts) encoding sgRNA targeting the homology junction was used. Not inserted in the genome. |
| Phenotypic information | Homozygous:Appear and breed normally. Live to at least one year of age.Heterozygous:Appear and breed normally. Live to at least one year of age. |
| Breeding history | Chimeras generated from V6.5 ESCs were bred to C57BL/6N mice, and progeny further backcrossed for a total of 7 generations. A SNP analysis indicated the genetic background to be approximately 97.5% C57BL/6N. They are routinely maintained as homozygotes. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
Literature references
- Tagger-A Swiss army knife for multiomics to dissect cell type-specific mechanisms of gene expression in mice.;Kaczmarczyk Lech, Bansal Vikas, Rajput Ashish, Rahman Raza-Ur, Krzyżak Wiesław, Degen Joachim, Poll Stefanie, Fuhrmann Martin, Bonn Stefan, Jackson Walker Scot, ;2019;PLoS biology;17;e3000374; 31393866