B6J.129P2-Rr27tm2Wrk/H
| Status | Available to order |
| EMMA ID | EM:02218 |
| International strain name | B6J.129P2-Rr27tm2Wrk/H |
| Alternative name | Delta K, H19 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Rr27tm2Wrk, |
| Gene/Transgene symbol | Rr27 |
Information from provider
| Provider | Wolf Reik |
| Provider affiliation | Laboratory of Imprinting and Development Genetics |
| Genetic information | A 1.7 kb direct tandem repeat unit in the differentially methylated region (DMR, the K repeat) was replaced with a floxed neo cassette, subsequently excised via transiently expressed Cre mediated recombination. Southern blot confirmed recombination. Northern blot showed no significant difference in gene expression between mutant and wild type tissues. |
| Phenotypic information | Deletion of the Rr27/H19 transcription start site shows no effect on imprinting expression or DNA methylation. |
| References |
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Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Tandem repeat hypothesis in imprinting: deletion of a conserved direct repeat element upstream of H19 has no effect on imprinting in the Igf2-H19 region.;Lewis Annabelle, Mitsuya Kohzoh, Constancia Miguel, Reik Wolf, ;2004;Molecular and cellular biology;24;5650-6; 15199123