MGI phenotypes (gene matching)
B6J.129P2-Rr27tm2Wrk/H
| Status | Available to order |
| EMMA ID | EM:02218 |
| Citation information | RRID:IMSR_EM:02218 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6J.129P2-Rr27tm2Wrk/H |
| Alternative name | Delta K, H19 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Rr27tm2Wrk |
| Gene/Transgene symbol | Rr27 |
Information from provider
| Provider | Wolf Reik |
| Provider affiliation | Laboratory of Imprinting and Development Genetics |
| Genetic information | A 1.7 kb direct tandem repeat unit in the differentially methylated region (DMR, the K repeat) was replaced with a floxed neo cassette, subsequently excised via transiently expressed Cre mediated recombination. Southern blot confirmed recombination. Northern blot showed no significant difference in gene expression between mutant and wild type tissues. |
| Phenotypic information | Deletion of the Rr27/H19 transcription start site shows no effect on imprinting expression or DNA methylation. |
| References |
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Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Disease and phenotype information
Literature references
- Tandem repeat hypothesis in imprinting: deletion of a conserved direct repeat element upstream of H19 has no effect on imprinting in the Igf2-H19 region.;Lewis Annabelle, Mitsuya Kohzoh, Constancia Miguel, Reik Wolf, ;2004;Molecular and cellular biology;24;5650-6; 15199123