B6;CBA-Tg(Igf2/LacZ,H19)YZ8Aco/AcoH
| Status | Available to order |
| EMMA ID | EM:02381 |
| International strain name | B6;CBA-Tg(Igf2/LacZ,H19)YZ8Aco/AcoH |
| Alternative name | YZ8 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Igf2/LacZ,H19)YZ8Aco |
| Gene/Transgene symbol | Tg(Igf2/LacZ,H19)YZ8Aco |
Information from provider
| Provider | Justin Ainscough |
| Provider affiliation | Central Biomedical Services, University of Leeds |
| Genetic information | The YZ8 line (single copy transgene) contains an additional 130 kb genomic region from mouse distal chromosome 7 spanning from Igf2 to ~40 kb downstream of H19. Igf2 has been inactivated by insertional mutagenesis resulting in an Igf2-LacZ gene fusion. LacZ activity shows spatial expression of Igf2 in a subset of tissues. The imprinted H19 gene is only expressed when transmitted through the maternal germline. After paternal transmission the gene is repressed. The Igf2-LacZ gene shows appropriate reciprocal imprinted behaviour (paternal allele specific expression, maternal repression). |
| Phenotypic information | The line has not been kept homozygous due to reduced fertility. |
| Breeding history | Brother sister matings with sporadic backcrossing to C57BL/6. |
| References |
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Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Imprinting of Igf2 and H19 from a 130 kb YAC transgene.;Ainscough J F, Koide T, Tada M, Barton S, Surani M A, ;1997;Development (Cambridge, England);124;3621-32; 9342054
- Appropriate expression of the mouse H19 gene utilises three or more distinct enhancer regions spread over more than 130 kb.;Ainscough J F, Dandolo L, Surani M A, ;2000;Mechanisms of development;91;365-8; 10704866