- hyperactivity / IMPC
- increased vertical activity / IMPC
- increased startle reflex / IMPC
- improved glucose tolerance / IMPC
- abnormal retina inner nuclear layer morphology / IMPC
- decreased startle reflex / IMPC
- increased circulating creatinine level / IMPC
- decreased total retina thickness / IMPC
- abnormal contextual conditioning behavior / IMPC
- short tibia / IMPC
- increased bone mineral content / IMPC
- abnormal heart left ventricle morphology / IMPC
- limb grasping / IMPC
- preweaning lethality, incomplete penetrance / IMPC
- increased bone mineral density / IMPC
- decreased body length / IMPC
- decreased total body fat amount / IMPC
- increased blood urea nitrogen level / IMPC
- abnormal bone structure / IMPC
- increased hematocrit / IMPC
- decreased grip strength / IMPC
- increased circulating alkaline phosphatase level / IMPC
- decreased exploration in new environment / IMPC
STOCK Thratm4.1Ven/Kctt
Status | Available to order |
EMMA ID | EM:04369 |
Citation information | RRID:IMSR_EM:04369 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
International strain name | STOCK Thratm4.1Ven/Kctt |
Alternative name | TRa1-GFP(ven) |
Strain type | Targeted Mutant Strains : Knock-in |
Allele/Transgene symbol | Thratm4.1Ven |
Gene/Transgene symbol | Thra |
Information from provider
Provider | Björn Vennström |
Provider affiliation | Department of Cell and Molecular Biology, Karolinska Institutet, CMB |
Genetic information | A coding sequence for the enhanced green fluorescent protein (EGFP) was inserted in frame by homologous recombination in ES cell 3' to the coding sequence for thyroid hormone receptor alpha 1 (TRa1). In vitro, the TRa1-GFP chimeric protein transactivates reporter genes in transfection experiments with an efficiency similar to that of the wt receptor. In the genetically modified mice, the targeted allele is transcribed into a RNA of a size expected from the genetic alteration; however, no RNA with the potential to encode the TRa2 isoform is generated. |
Phenotypic information | The heterozygous and homozygous mice express a TRa1-GFP chimeric protein, which localizes to the nucleus in the mouse. Both the heterozygous and homozygous mice show a normal phenotype. No alterations have been detected in the following parameters: - levels of T3 and T4 in serum - postnatal growth rate - lean and fat body mass - organ weights - behavior as assayed by the SHIRPA primary test The mice are quite useful for detecting expression of TRa1 in mouse tissues, especially as no/few reliable antibodies are available. |
Breeding history | Chimeric founder mice were bred against C57BL/6NCrl and the offspring was subsequently crossed with EIIa-Cre mice for removal of the Neo-cassette. Heterozygote offspring was intercrossed to generate mice homozygote for the TRa1-GFP protein or backcrossed against C57BL/6 for three generations and then intercrossed. |
References |
|
Homozygous fertile | yes |
Homozygous viable | yes |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | Karolinska Institutet, Stockholm, Sweden |
Animals used for archiving | homozygous males, wild-type C57BL/6NCrl females |
Breeding at archiving centre | No breeding. |
Stage of embryos | 2-cell |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Resistance to thyroid hormone due to a mutation in thyroid hormone receptor alpha / Orphanet_566231
IMPC phenotypes (gene matching)
MGI phenotypes (gene matching)
- decreased bone mineral density / MGI
- abnormal trabecular bone morphology / MGI
- abnormal long bone metaphysis morphology / MGI
- decreased compact bone thickness / MGI
- delayed hair appearance / MGI
- abnormal enterocyte morphology / MGI
- abnormal small intestine morphology / MGI
- abnormal intestinal mucosa morphology / MGI
- short limbs / MGI
- short tail / MGI
- abnormal hippocampus morphology / MGI
- abnormal cerebellum external granule cell layer morphology / MGI
- decreased body length / MGI
- increased body weight / MGI
- obese / MGI
- decreased body weight / MGI
- increased anxiety-related response / MGI
- abnormal pup retrieval / MGI
- abnormal object recognition memory / MGI
- abnormal osteoclast physiology / MGI
- abnormal bone strength / MGI
- abnormal postnatal growth / MGI
- postnatal growth retardation / MGI
- reduced fertility / MGI
- reduced male fertility / MGI
- reduced female fertility / MGI
- decreased litter size / MGI
- abnormal glucose homeostasis / MGI
- increased circulating insulin level / MGI
- premature death / MGI
- small heart / MGI
- increased heart rate / MGI
- increased glucagon secretion / MGI
- increased circulating glucagon level / MGI
- short tibia / MGI
- abnormal thyroid physiology / MGI
- increased insulin sensitivity / MGI
- abnormal bone mineralization / MGI
- abnormal joint morphology / MGI
- abnormal bone remodeling / MGI
- abnormal long bone epiphyseal plate morphology / MGI
- increased insulin secretion / MGI
- prolonged QT interval / MGI
- abnormal locomotor coordination / MGI
- decreased liver weight / MGI
- decreased width of hypertrophic chondrocyte zone / MGI
- delayed endochondral bone ossification / MGI
- delayed intramembranous bone ossification / MGI
- abnormal intestinal goblet cell morphology / MGI
- thyroid hypoplasia / MGI
- increased activity of thyroid / MGI
- decreased activity of thyroid / MGI
- abnormal insulin secretion / MGI
- oliguria / MGI
- abnormal response/metabolism to endogenous compounds / MGI
- abnormal long bone epiphyseal plate proliferative zone / MGI
- abnormal bone structure / MGI
- abnormal compact bone morphology / MGI
- abnormal defecation / MGI
- abnormal hormone level / MGI
- abnormal thyroid-stimulating hormone level / MGI
- increased compact bone thickness / MGI
- abnormal long bone diaphysis morphology / MGI
- short ulna / MGI
- abnormal cochlear outer hair cell morphology / MGI
- decreased length of long bones / MGI
- abnormal thyroid follicle morphology / MGI
- decreased circulating insulin-like growth factor I level / MGI
- decreased mean systemic arterial blood pressure / MGI
- decreased osteoclast cell number / MGI
- decreased bone resorption / MGI
- increased circulating thyroid-stimulating hormone level / MGI
- increased thyroid-stimulating hormone level / MGI
- abnormal pancreatic islet morphology / MGI
- impaired glucose tolerance / MGI
- insulin resistance / MGI
- decreased heart rate / MGI
- hearing/vestibular/ear phenotype / MGI
- behavior/neurological phenotype / MGI
- skeleton phenotype / MGI
- osteosclerosis / MGI
- increased triiodothyronine level / MGI
- increased circulating thyroxine level / MGI
- decreased circulating thyroxine level / MGI
- decreased circulating triiodothyronine level / MGI
- increased circulating triiodothyronine level / MGI
- increased body temperature / MGI
- decreased body temperature / MGI
- increased circulating glucose level / MGI
- abnormal enzyme/coenzyme activity / MGI
- decreased ventricle muscle contractility / MGI
- increased bone mass / MGI
- increased circulating leptin level / MGI
- increased long bone epiphyseal plate size / MGI
- abnormal long bone epiphyseal ossification zone morphology / MGI
- impaired lipolysis / MGI
- increased diameter of long bones / MGI
- abnormal enterocyte physiology / MGI
- abnormal enterocyte proliferation / MGI
- prolonged estrous cycle / MGI
- small pancreatic islets / MGI
- increased epididymal fat pad weight / MGI
- increased trabecular bone thickness / MGI
- abnormal chondrocyte physiology / MGI
- increased total body fat amount / MGI
- abnormal bone mineral content / MGI
- increased bone mineral content / MGI
- decreased bone mineral content / MGI
- prolonged QRS complex duration / MGI
- delayed cranial suture closure / MGI
- increased bone trabecula number / MGI
- increased trabecular bone volume / MGI
- abnormal mitochondrial ATP synthesis coupled electron transport / MGI
- increased compact bone volume / MGI
- decreased compact bone area / MGI
- decreased core body temperature / MGI
- impaired adaptive thermogenesis / MGI
- postnatal lethality, complete penetrance / MGI
- perinatal lethality, incomplete penetrance / MGI
- prenatal lethality, complete penetrance / MGI
- decreased food intake / MGI
- decreased areal bone mineral density / MGI
- decreased small intestine length / MGI
- decreased small intestinal villus height / MGI
- decreased cardiac cell glucose uptake / MGI
- wide cranial sutures / MGI
- large fontanelles / MGI
Literature references
- The thyroid hormone receptor alpha1 protein is expressed in embryonic postmitotic neurons and persists in most adult neurons.;Wallis Karin, Dudazy Susi, van Hogerlinden Max, Nordström Kristina, Mittag Jens, Vennström Björn, ;2010;Molecular endocrinology (Baltimore, Md.);24;1904-16; 20739404
- Unliganded thyroid hormone receptor alpha1 impairs adult hippocampal neurogenesis.;Kapoor Richa, van Hogerlinden Max, Wallis Karin, Ghosh Himanish, Nordstrom Kristina, Vennstrom Bjorn, Vaidya Vidita A, ;2010;FASEB journal : official publication of the Federation of American Societies for Experimental Biology;24;4793-805; 20709911
- Identification of thyroid hormone response elements in vivo using mice expressing a tagged thyroid hormone receptor α1.;Dudazy-Gralla Susi, Nordström Kristina, Hofmann Peter Josef, Meseh Dina Abdul, Schomburg Lutz, Vennström Björn, Mittag Jens, ;2013;Bioscience reports;33;e00027; 23398480
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