MGI phenotypes (allele matching)
STOCK Thratm4.1Ven/Kctt
| Status | Available to order |
| EMMA ID | EM:04369 |
| International strain name | STOCK Thratm4.1Ven/Kctt |
| Alternative name | TRa1-GFP(ven) |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Thratm4.1Ven, |
| Gene/Transgene symbol | Thra |
Information from provider
| Provider | Björn Vennström |
| Provider affiliation | Department of Cell and Molecular Biology, Karolinska Institutet, CMB |
| Genetic information | A coding sequence for the enhanced green fluorescent protein (EGFP) was inserted in frame by homologous recombination in ES cell 3' to the coding sequence for thyroid hormone receptor alpha 1 (TRa1). In vitro, the TRa1-GFP chimeric protein transactivates reporter genes in transfection experiments with an efficiency similar to that of the wt receptor. In the genetically modified mice, the targeted allele is transcribed into a RNA of a size expected from the genetic alteration; however, no RNA with the potential to encode the TRa2 isoform is generated. |
| Phenotypic information | The heterozygous and homozygous mice express a TRa1-GFP chimeric protein, which localizes to the nucleus in the mouse. Both the heterozygous and homozygous mice show a normal phenotype. No alterations have been detected in the following parameters: - levels of T3 and T4 in serum - postnatal growth rate - lean and fat body mass - organ weights - behavior as assayed by the SHIRPA primary test The mice are quite useful for detecting expression of TRa1 in mouse tissues, especially as no/few reliable antibodies are available. |
| Breeding history | Chimeric founder mice were bred against C57BL/6NCrl and the offspring was subsequently crossed with EIIa-Cre mice for removal of the Neo-cassette. Heterozygote offspring was intercrossed to generate mice homozygote for the TRa1-GFP protein or backcrossed against C57BL/6 for three generations and then intercrossed. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Karolinska Institutet, Stockholm, Sweden |
| Animals used for archiving | homozygous 0, wild-type C57BL/6NCrl |
| Breeding at archiving centre | No breeding. |
| Stage of embryos | 2-cell |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Resistance to thyroid hormone due to a mutation in thyroid hormone receptor alpha / Orphanet_566231
Literature references
- The thyroid hormone receptor alpha1 protein is expressed in embryonic postmitotic neurons and persists in most adult neurons.;Wallis Karin, Dudazy Susi, van Hogerlinden Max, Nordström Kristina, Mittag Jens, Vennström Björn, ;2010;Molecular endocrinology (Baltimore, Md.);24;1904-16; 20739404
- Unliganded thyroid hormone receptor alpha1 impairs adult hippocampal neurogenesis.;Kapoor Richa, van Hogerlinden Max, Wallis Karin, Ghosh Himanish, Nordstrom Kristina, Vennstrom Bjorn, Vaidya Vidita A, ;2010;FASEB journal : official publication of the Federation of American Societies for Experimental Biology;24;4793-805; 20709911