STOCK Fzr1tm1Mama/Cnbc
| Status | Available to order |
| EMMA ID | EM:06108 |
| International strain name | STOCK Fzr1tm1Mama/Cnbc |
| Alternative name | Frz1 |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Fzr1tm1Mama, |
| Gene/Transgene symbol | Fzr1 |
Information from provider
| Provider | Marcos Malumbres |
| Provider affiliation | Molecular Oncology, Centro Nacional de Investigaciones Oncologicas (CNIO) |
| Genetic information | A loxP site was inserted upstream of exon 2 and an frt-flanked neo cassette with a 5' loxP site was inserted downstream of exon 3. Germ line, flp-mediated recombination was used to remove the neo cassette leaving exons 2 and 3 floxed; cre-mediated recombination between the two loxP sites eliminates exons 2 and 3. Splicing from exon 1 to exon 4 sequences causes a frameshift in the open reading frame that results in the synthesis of a putative small polypeptide unrelated to Fzr1. |
| Phenotypic information | Before cre-mediated recombination there is no phenotype associated to this allele. |
| Breeding history | Chimeras were crossed with C57BL/6J females for germ line transmission testing. To remove the neoR selection marker Fzr1+/loxfrt mice were crossed with Tg(ACTFLPe)9205Dym mice to remove the frt-flanked neomycin resistance cassette used for homologous recombination selection. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous mixed |
Literature references
- Genomic stability and tumour suppression by the APC/C cofactor Cdh1.;García-Higuera Irene, Manchado Eusebio, Dubus Pierre, Cañamero Marta, Méndez Juan, Moreno Sergio, Malumbres Marcos, ;2008;Nature cell biology;10;802-11; 18552834
- The APC/C activator FZR1 coordinates the timing of meiotic resumption during prophase I arrest in mammalian oocytes.;Holt Janet E, Tran Suzanne M-T, Stewart Jessica L, Minahan Kyra, García-Higuera Irene, Moreno Sergio, Jones Keith T, ;2011;Development (Cambridge, England);138;905-13; 21270054