CBAB6-Tg(Tek-Nfkbia*S32A*S36A)1Gkl/Flmg
Status | Available to order |
EMMA ID | EM:07265 |
Citation information | RRID:IMSR_EM:07265 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
International strain name | CBAB6-Tg(Tek-Nfkbia*S32A*S36A)1Gkl/Flmg |
Alternative name | CBA.B6-Tg(Tie2-IkB)1Gkl/Flmg |
Strain type | Transgenic Strains |
Allele/Transgene symbol | Tg(Tek-Nfkbia*S32A*S36A)1Gkl |
Gene/Transgene symbol | Tg(Tek-Nfkbia*S32A*S36A)1Gkl |
Information from provider
Provider | George Kollias |
Provider affiliation | Institute of Immunology, B.S.R.C. Al. Fleming |
Genetic information | The pLHLCA-HA1 vector was opened by MluI, filled-in and digested by HindIII. The resulting fragment was ligated into the linearized, by EcoRV/HindIII, beta-globin intron producing intron-IkappaBalpha construct. A 5,3 Kb XbaI/XbaI fragment from beta-Gal stop floxed was ligated into the SpeI site of intron-IkappaBalpha, 5' of the intronic sequence, generating the floxedstop-IkappaBalpha vector. A SalI/XhoI fragment of FRT site was inserted into the SalI site of floxedstop-IkappaBalpha. A SalI filled-in/NotI filled-in fragment from the resulting plasmid was then ligated in the SmaI cloning site of Tie2 to obtain the final Tie2floxedstop-IkappaBalpha construct. Transgenic mice were generated by microinjection of the linear 19Kb Tie2floxedstop-IkappaBalpha construct into fertilized C57BL/6 x CBA oocytes. Founders were selected by Southern Blot analysis. |
Phenotypic information | Tie2DNIkappaBalpha transgenic mice express an HA-tagged murine IkappaBalpha cDNA with alanine substitutions of serines 32 and 36 (HA-IkappaBalpha [A32/A36]), specifically in endothelial cells. |
Breeding history | Litters are routinely screened by PCR analysis of tail DNA. Transgenic lineages were maintained heterozygous by crossing them to C57BL/6-CBA wild-type mice. |
References |
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Homozygous fertile | not known |
Homozygous viable | not known |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | B.S.R.C. Alexander Fleming, Vari, Greece |
Animals used for archiving | heterozygous males |
Literature references
- Endothelial cell-specific NF-kappaB inhibition protects mice from atherosclerosis.;Gareus Ralph, Kotsaki Elena, Xanthoulea Sofia, van der Made Ingeborg, Gijbels Marion J J, Kardakaris Rozina, Polykratis Apostolos, Kollias George, de Winther Menno P J, Pasparakis Manolis, ;2008;Cell metabolism;8;372-83; 19046569
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