CBAB6-Tg(Tek-Nfkbia*S32A*S36A)1Gkl/Flmg
| Status | Available to order |
| EMMA ID | EM:07265 |
| International strain name | CBAB6-Tg(Tek-Nfkbia*S32A*S36A)1Gkl/Flmg |
| Alternative name | CBA.B6-Tg(Tie2-IkB)1Gkl/Flmg |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Tek-Nfkbia*S32A*S36A)1Gkl, |
| Gene/Transgene symbol | Tg(Tek-Nfkbia*S32A*S36A)1Gkl |
Information from provider
| Provider | George Kollias |
| Provider affiliation | Institute of Immunology, B.S.R.C. Al. Fleming |
| Genetic information | The pLHLCA-HA1 vector was opened by MluI, filled-in and digested by HindIII. The resulting fragment was ligated into the linearized, by EcoRV/HindIII, beta-globin intron producing intron-IkappaBalpha construct. A 5,3 Kb XbaI/XbaI fragment from beta-Gal stop floxed was ligated into the SpeI site of intron-IkappaBalpha, 5' of the intronic sequence, generating the floxedstop-IkappaBalpha vector. A SalI/XhoI fragment of FRT site was inserted into the SalI site of floxedstop-IkappaBalpha. A SalI filled-in/NotI filled-in fragment from the resulting plasmid was then ligated in the SmaI cloning site of Tie2 to obtain the final Tie2floxedstop-IkappaBalpha construct. Transgenic mice were generated by microinjection of the linear 19Kb Tie2floxedstop-IkappaBalpha construct into fertilized C57BL/6 x CBA oocytes. Founders were selected by Southern Blot analysis. |
| Phenotypic information | Tie2DNIkappaBalpha transgenic mice express an HA-tagged murine IkappaBalpha cDNA with alanine substitutions of serines 32 and 36 (HA-IkappaBalpha [A32/A36]), specifically in endothelial cells. |
| Breeding history | Litters are routinely screened by PCR analysis of tail DNA. Transgenic lineages were maintained heterozygous by crossing them to C57BL/6-CBA wild-type mice. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | B.S.R.C. Alexander Fleming, Vari, Greece |
| Animals used for archiving | heterozygous 0 |
Literature references
- Endothelial cell-specific NF-kappaB inhibition protects mice from atherosclerosis.;Gareus Ralph, Kotsaki Elena, Xanthoulea Sofia, van der Made Ingeborg, Gijbels Marion J J, Kardakaris Rozina, Polykratis Apostolos, Kollias George, de Winther Menno P J, Pasparakis Manolis, ;2008;Cell metabolism;8;372-83; 19046569