C57BL/6N-Nxntm1b(EUCOMM)Wtsi/Ieg

Status

Available to order

EMMA IDEM:08100
International strain nameC57BL/6N-Nxntm1b(EUCOMM)Wtsi/Ieg
Alternative nameEPD0045_1_B11
Strain typeTargeted Mutant Strains
Allele/Transgene symbolNxntm1b(EUCOMM)Wtsi
Gene/Transgene symbolNxn
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliationInstitute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0045_1_B11. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele (Gt(ROSA)26Sor). Click here for more information on EUCOMM final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivingheterozygous C57BL/6NTacUSA

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (allele matching)
  • cleft palate / IMPC
  • abnormal craniofacial morphology / IMPC
  • microcephaly / IMPC
  • abnormal cranium morphology / IMPC
  • abnormal snout morphology / IMPC
  • syndactyly / IMPC
  • abnormal mammary gland morphology / IMPC
  • abnormal eyelid morphology / IMPC
  • abnormal locomotor behavior / IMPC
  • decreased locomotor activity / IMPC
  • no spontaneous movement / IMPC
  • decreased exploration in new environment / IMPC
  • unresponsive to tactile stimuli / IMPC
  • increased circulating triglyceride level / IMPC
  • increased circulating HDL cholesterol level / IMPC
  • abnormal embryo size / IMPC
  • abnormal digit morphology / IMPC
  • short tibia / IMPC
  • small adrenal glands / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • abnormal facial morphology / IMPC
  • abnormal optic disk morphology / IMPC
  • facial cleft / IMPC
  • increased effector memory CD8-positive, alpha-beta T cell number / IMPC
  • preweaning lethality, complete penetrance / IMPC
  • abnormal head shape / IMPC
  • abnormal head size / IMPC
  • abnormal freezing behavior / IMPC
IMPC phenotypes (gene matching)
  • abnormal embryo size / IMPC
  • cleft palate / IMPC
  • unresponsive to tactile stimuli / IMPC
  • abnormal facial morphology / IMPC
  • abnormal craniofacial morphology / IMPC
  • facial cleft / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • decreased cardiac muscle contractility / IMPC
  • increased grip strength / IMPC
  • abnormal head size / IMPC
  • increased mean corpuscular hemoglobin concentration / IMPC
  • preweaning lethality, complete penetrance / IMPC
  • abnormal head shape / IMPC
  • small adrenal glands / IMPC
  • decreased locomotor activity / IMPC
  • abnormal freezing behavior / IMPC
  • decreased body length / IMPC
  • abnormal eyelid morphology / IMPC
  • abnormal digit morphology / IMPC
  • abnormal optic disk morphology / IMPC
  • abnormal locomotor behavior / IMPC
  • increased circulating bilirubin level / IMPC
  • microcephaly / IMPC
  • short tibia / IMPC
  • abnormal mammary gland morphology / IMPC
  • anophthalmia / IMPC
  • abnormal retina blood vessel morphology / IMPC
  • irregularly shaped pupil / IMPC
  • syndactyly / IMPC
  • abnormal cranium morphology / IMPC
  • preweaning lethality, incomplete penetrance / IMPC
  • increased effector memory CD8-positive, alpha-beta T cell number / IMPC
  • decreased exploration in new environment / IMPC
  • abnormal behavior / IMPC
  • no spontaneous movement / IMPC
  • increased fasting circulating glucose level / IMPC
  • abnormal snout morphology / IMPC
  • increased circulating HDL cholesterol level / IMPC
  • increased circulating triglyceride level / IMPC
MGI phenotypes (allele matching)
  • cleft palate / MGI
  • double outlet right ventricle / MGI
  • enlarged liver sinusoidal spaces / MGI
  • abnormal Mullerian duct morphology / MGI
  • absent stapedial artery / MGI
  • perimembraneous ventricular septal defect / MGI
  • herniated liver / MGI
  • decreased intestine length / MGI
  • abnormal vitelline vein topology / MGI
MGI phenotypes (gene matching)
  • cleft palate / MGI
  • abnormal sternum morphology / MGI
  • double outlet right ventricle / MGI
  • enlarged liver sinusoidal spaces / MGI
  • persistent truncus arteriosis / MGI
  • abnormal Mullerian duct morphology / MGI
  • decreased fetal size / MGI
  • small frontal bone / MGI
  • absent stapedial artery / MGI
  • split xiphoid process / MGI
  • increased osteoblast cell number / MGI
  • abnormal cell physiology / MGI
  • ventricular septal defect / MGI
  • atrioventricular septal defect / MGI
  • perimembraneous ventricular septal defect / MGI
  • herniated liver / MGI
  • perinatal lethality, complete penetrance / MGI
  • decreased intestine length / MGI
  • abnormal vitelline vein topology / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.


Search
INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).